ARF1

Adp-ribosylation factor 1

Reviewed UniProt entries for ARF1 and its protein partners


UniProt code UniProt Name Protein Name Gene Name Organism Length
P36397 ARF1_ARATH ADP-ribosylation factor 1 (AtARF1) ARF1 At2g47170 T3D7.2 T8I13.1 Arabidopsis thaliana (Mouse-ear cress) 181
P84080 ARF1_BOVIN ADP-ribosylation factor 1 ARF1 Bos taurus (Bovine) 181
Q96361 ARF1_BRARP ADP-ribosylation factor 1 ARF1 Brassica rapa subsp. pekinensis (Chinese cabbage) (Brassica pekinensis) 182
O23778 ARF1_CATRO ADP-ribosylation factor 1 ARF1 Catharanthus roseus (Madagascar periwinkle) (Vinca rosea) 181
P51821 ARF1_CHLRE ADP-ribosylation factor 1 ARF1 Chlamydomonas reinhardtii (Chlamydomonas smithii) 181
P51822 ARF1_DAUCA ADP-ribosylation factor 1 ARF1 Daucus carota (Carrot) 181
O00909 ARF1_DICDI ADP-ribosylation factor 1 arfA arf1 DDB_G0289173 Dictyostelium discoideum (Slime mold) 182
P61209 ARF1_DROME ADP-ribosylation factor 1 Arf79F ARF1 CG8385 Drosophila melanogaster (Fruit fly) 182
P84077 ARF1_HUMAN ADP-ribosylation factor 1 ARF1 Homo sapiens (Human) 181
P61210 ARF1_LOCMI ADP-ribosylation factor 1 (lARF1) ARF1 Locusta migratoria (Migratory locust) 182
Q4R5P2 ARF1_MACFA ADP-ribosylation factor 1 ARF1 QccE-11717 Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) 181
P84078 ARF1_MOUSE ADP-ribosylation factor 1 Arf1 Mus musculus (Mouse) 181
Q7KQL3 ARF1_PLAF7 ADP-ribosylation factor 1 (pfARF1) ARF1 ARF PF10_0203 Plasmodium falciparum (isolate 3D7) 181
Q94650 ARF1_PLAFA ADP-ribosylation factor 1 (plARF) ARF1 ARF Plasmodium falciparum 181
Q25761 ARF1_PLAFO ADP-ribosylation factor 1 ARF1 Plasmodium falciparum (isolate NF54) 181
P84079 ARF1_RAT ADP-ribosylation factor 1 Arf1 Rattus norvegicus (Rat) 181
O48649 ARF1_SALBA ADP-ribosylation factor 1 ARF1 Salix bakko (Japanese willow) 181
P36579 ARF1_SCHPO ADP-ribosylation factor 1 arf1 SPBC4F6.18c Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) 180
P51643 ARF1_XENLA ADP-ribosylation factor 1 arf1 Xenopus laevis (African clawed frog) 181
P11076 ARF1_YEAST ADP-ribosylation factor 1 ARF1 YDL192W D1244 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Bakers yeast) 181
Q2KI41 CYH2_BOVIN Cytohesin-2 (PH; SEC7 and coiled-coil domain-containing protein 2) CYTH2 PSCD2 Bos taurus (Bovine) 410
Q76MY7 CYH2_CHLAE Cytohesin-2 (PH; SEC7 and coiled-coil domain-containing protein 2) CYTH2 PSCD2 Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops) 399
Q99418 CYH2_HUMAN Cytohesin-2 (ARF exchange factor) (ARF nucleotide-binding site opener) (Protein ARNO) (PH; SEC7 and coiled-coil domain-containing protein 2) CYTH2 ARNO PSCD2 PSCD2L Homo sapiens (Human) 400
P63034 CYH2_MOUSE Cytohesin-2 (PH; SEC7 and coiled-coil domain-containing protein 2) (CLM2) (SEC7 homolog B) (mSec7-2) Cyth2 Pscd2 Sec7b Mus musculus (Mouse) 400
P63035 CYH2_RAT Cytohesin-2 (ARF nucleotide-binding site opener) (Protein ARNO) (PH; SEC7 and coiled-coil domain-containing protein 2) (CLM2) (SEC7 homolog B) Cyth2 Pscd2 Sec7b Rattus norvegicus (Rat) 400

Small molecules tested in ARF1 binding assays:

4 datapoints for 2 distinct small molecules


Name Brefeldin A
Affinity None
Assay description None
-- Direct single protein target assigned: ARF1_BOVIN (Expert curation) --
DOI http://www.nature.com/nature/journal/v426/n6966/full/nature02197.html
Doc title Structural snapshots of the mechanism and inhibition of a guanine nucleotide exchange factor
PDB 1R8Q - (AFB)

Name CHEMBL401604
Affinity Activity = 49.7uM
Assay description Inhibition of wild type ARNO activation
-- Homologous single protein target assigned: CYH2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/17563369
Doc title Structure-based discovery of an inhibitor of Arf activation by Sec7 domains through targeting of protein-protein complexes.
PDB None

Name CHEMBL401604
Affinity Kapp = 0.0078/s
Assay description Reduction in nucleotide exchange on Arf1 using ARNO4M
-- Homologous single protein target assigned: ARF1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/17563369
Doc title Structure-based discovery of an inhibitor of Arf activation by Sec7 domains through targeting of protein-protein complexes.
PDB None

Name CHEMBL401604
Affinity Active
Assay description Inhibition of ARNO-stimulated nucleotide exchange of Arf1
-- Homologous single protein target assigned: ARF1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/17563369
Doc title Structure-based discovery of an inhibitor of Arf activation by Sec7 domains through targeting of protein-protein complexes.
PDB None

19 PDB entries for ARF1 and its protein partners


1HUR
Structure of the human ADP-ribosylation factor 1 complexed with GDP.

Entity type Code Description
polypeptide(L) P84077 HUMAN ADP-RIBOSYLATION FACTOR 1
Ligand GDP GUANOSINE-5'-DIPHOSPHATE

1O3Y
Molecular mechanism of membrane recruitment of GGA by ARF in lysosomal protein transport

Entity type Code Description
polypeptide(L) P84078 ADP-ribosylation factor 1
Ligand GTP GUANOSINE-5'-TRIPHOSPHATE

1PBV
Structure of the Sec7 domain of the Arf exchange factor ARNO.

Entity type Code Description
polypeptide(L) Q99418 ARNO

1R8M
Structural snapshots of the mechanism and inhibition of a guanine nucleotide exchange factor

Entity type Code Description
polypeptide(L) Q99418 Arno

1R8Q
Structural snapshots of the mechanism and inhibition of a guanine nucleotide exchange factor

Entity type Code Description
polypeptide(L) P84080 ADP-ribosylation factor 1
polypeptide(L) Q99418 Arno
Ligand G3D GUANOSINE-3'-MONOPHOSPHATE-5'-DIPHOSPHATE
Ligand AFB 1,6,7,8,9,11A,12,13,14,14A-DECAHYDRO-1,13-DIHYDROXY-6-METHYL-4H-CYCLOPENT[F]OXACYCLOTRIDECIN-4-ONE

1R8S
Structural snapshots of the mechanism and inhibition of a guanine nucleotide exchange factor

Entity type Code Description
polypeptide(L) P84080 ADP-ribosylation factor 1
polypeptide(L) Q99418 Arno
Ligand SO3 SULFITE ION
Ligand GDP GUANOSINE-5'-DIPHOSPHATE
Ligand BME BETA-MERCAPTOETHANOL

1RRF
The structure of rat ADP-ribosylation factor-1 (ARF-1) complexed to GDP determined from two different crystal forms.

Entity type Code Description
polypeptide(L) P84079 RAT ADP-RIBOSYLATION FACTOR-1
Ligand GDP GUANOSINE-5'-DIPHOSPHATE

1RRG
The structure of rat ADP-ribosylation factor-1 (ARF-1) complexed to GDP determined from two different crystal forms.

Entity type Code Description
polypeptide(L) P84079 RAT ADP-RIBOSYLATION FACTOR-1
Ligand GDP GUANOSINE-5'-DIPHOSPHATE

1S9D
Structural Snapshots of the Mechanism and Inhibition of a Guanine Nucleotide Exchange Factor

Entity type Code Description
polypeptide(L) P84080 ADP-Ribosylation Factor 1
polypeptide(L) Q99418 Arno
Ligand GDP GUANOSINE-5'-DIPHOSPHATE
Ligand AFB 1,6,7,8,9,11A,12,13,14,14A-DECAHYDRO-1,13-DIHYDROXY-6-METHYL-4H-CYCLOPENT[F]OXACYCLOTRIDECIN-4-ONE

1U27
Structural determinants of phosphoinositide selectivity in splice variants of Grp1 family PH domains

Entity type Code Description
polypeptide(L) P63034 Cytohesin 2
Ligand 4IP INOSITOL-(1,3,4,5)-TETRAKISPHOSPHATE

1U29
Structural determinants of phosphoinositide selectivity in splice variants of Grp1 family PH domains

Entity type Code Description
polypeptide(L) P63034 Cytohesin 2
Ligand I3P D-MYO-INOSITOL-1,4,5-TRIPHOSPHATE

1U81
Conformational changes in human Arf1 on nucleotide exchange and deletion of membrane-binding elements.

Entity type Code Description
polypeptide(L) P84077 ADP-ribosylation factor 1
Ligand GDP GUANOSINE-5'-DIPHOSPHATE

2J59
Structural Basis for Arf1-Mediated Recruitment of Arhgap21 to Golgi Membranes.

Entity type Code Description
polypeptide(L) 2J59 ADP-RIBOSYLATION FACTOR 1
polypeptide(L) P84078 ADP-RIBOSYLATION FACTOR 1
polypeptide(L) Q8NI19 RHO-GTPASE ACTIVATING PROTEIN 10
Ligand GTP GUANOSINE-5'-TRIPHOSPHATE
Ligand DIO 1,4-DIETHYLENE DIOXIDE

2K5U
Structure and Membrane Interaction of Myristoylated ARF1

Entity type Code Description
polypeptide(L) P11076 ADP-ribosylation factor 1
Ligand GDP GUANOSINE-5'-DIPHOSPHATE

2KSQ
Dynamic structure of membrane-anchored Arf*GTP.

Entity type Code Description
polypeptide(L) P11076 ADP-ribosylation factor 1
Ligand MTN S-[(1-OXYL-2,2,5,5-TETRAMETHYL-2,5-DIHYDRO-1H-PYRROL-3-YL)METHYL] METHANESULFONOTHIOATE
Ligand GTP GUANOSINE-5'-TRIPHOSPHATE

3AQ4
Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b

Entity type Code Description
polypeptide(L) P36397 ADP-ribosylation factor 1
Ligand GDP GUANOSINE-5'-DIPHOSPHATE

3LRP
Structure of Plasmodium falciparum ADP-ribosylation factor 1

Entity type Code Description
polypeptide(L) Q94650 ADP-ribosylation factor 1
Ligand GDP GUANOSINE-5'-DIPHOSPHATE

3O47
Crystal structure of ARFGAP1-ARF1 fusion protein

Entity type Code Description
polypeptide(L) Q8N6T3 ADP-ribosylation factor GTPase-activating protein 1, ADP-ribosylation factor 1
polypeptide(L) P84077 ADP-ribosylation factor GTPase-activating protein 1, ADP-ribosylation factor 1
Ligand GDP GUANOSINE-5'-DIPHOSPHATE

3TJZ
A structure-based mechanism for arf1-dependent recruitment of coatomer to membranes.

Entity type Code Description
polypeptide(L) P11076 ADP-ribosylation factor 1
polypeptide(L) P53620 Coatomer subunit gamma
polypeptide(L) P35604 Coatomer subunit zeta-1
Ligand GNP PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER