Beta-catenin

Catenin beta-1 (beta-catenin)

Reviewed UniProt entries for Beta-catenin and its protein partners


UniProt code UniProt Name Protein Name Gene Name Organism Length
Q0VCX4 CTNB1_BOVIN Catenin beta-1 (Beta-catenin) CTNNB1 Bos taurus (Bovine) 781
P35222 CTNB1_HUMAN Catenin beta-1 (Beta-catenin) CTNNB1 CTNNB OK/SW-cl.35 PRO2286 Homo sapiens (Human) 781
Q02248 CTNB1_MOUSE Catenin beta-1 (Beta-catenin) Ctnnb1 Catnb Mus musculus (Mouse) 781
Q9WU82 CTNB1_RAT Catenin beta-1 (Beta-catenin) Ctnnb1 Catnb Rattus norvegicus (Rat) 781
Q924A0 TF7L2_MOUSE Transcription factor 7-like 2 (HMG box transcription factor 4) (T-cell-specific transcription factor 4) (T-cell factor 4) (TCF-4) (mTCF-4) Tcf7l2 Tcf4 Mus musculus (Mouse) 459
P70062 T7L1A_XENLA Transcription factor 7-like 1-A (HMG box transcription factor 3-A) (TCF-3-A) (xTcf-3) tcf7l1-a tcf3 tcf3a Xenopus laevis (African clawed frog) 554
Q90ZB6 T7L1B_XENLA Transcription factor 7-like 1-B (HMG box transcription factor 3-B) (TCF-3-B) (xTcf-3) tcf7l1-b tcf3 tcf3b Xenopus laevis (African clawed frog) 551
P70063 T7L1C_XENLA Transcription factor 7-like 1-C (HMG box transcription factor 3-C) (TCF-3-C) (xTcf-3c) tcf7l1-c tcf3c Xenopus laevis (African clawed frog) 550
P70064 T7L1D_XENLA Transcription factor 7-like 1-D (HMG box transcription factor 3-D) (TCF-3-D) (xTcf-3d) tcf7l1-d tcf3d Xenopus laevis (African clawed frog) 550
Q9HCS4 TF7L1_HUMAN Transcription factor 7-like 1 (HMG box transcription factor 3) (TCF-3) TCF7L1 TCF3 Homo sapiens (Human) 588
Q9Z1J1 TF7L1_MOUSE Transcription factor 7-like 1 (HMG box transcription factor 3) (TCF-3) (mTCF-3) Tcf7l1 Tcf3 Mus musculus (Mouse) 584
Q6GL68 TF7L1_XENTR Transcription factor 7-like 1 (HMG box transcription factor 3) (TCF-3) tcf7l1 tcf3 Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) 553
Q9NQB0 TF7L2_HUMAN Transcription factor 7-like 2 (HMG box transcription factor 4) (T-cell-specific transcription factor 4) (T-cell factor 4) (TCF-4) (hTCF-4) TCF7L2 TCF4 Homo sapiens (Human) 619

Small molecules tested in Beta-catenin binding assays:

25 datapoints for 23 distinct small molecules


Name PNU-74654
Affinity Kd (ITC) 450nM
Assay description ITC
-- Direct single protein target assigned: CTNB1_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1002/prot.20955
Doc title Inhibition of protein-protein interactions: the discovery of druglike beta-catenin inhibitors by combining virtual and biophysical screening.
PDB None

Name PKF118-310
Affinity IC50 (ELISA) 800nM
Assay description ELISA with betaCatenin inmobilized adding Tcf; it could interact with both
-- Direct single protein target assigned: CTNB1_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1016/S1535-6108(03)00334-9
Doc title Small-molecule antagonists of the oncogenic Tcf/beta-catenin protein complex.
PDB None

Name PKF118-744
Affinity IC50 (ELISA) 2.4uM
Assay description ELISA with betaCatenin inmobilized adding Tcf; it could interact with both
-- Direct single protein target assigned: CTNB1_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1016/S1535-6108(03)00334-9
Doc title Small-molecule antagonists of the oncogenic Tcf/beta-catenin protein complex.
PDB None

Name CGP049090
Affinity IC50 (ELISA) 9uM
Assay description ELISA with betaCatenin inmobilized adding Tcf; it could interact with both
-- Direct single protein target assigned: CTNB1_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1016/S1535-6108(03)00334-9
Doc title Small-molecule antagonists of the oncogenic Tcf/beta-catenin protein complex.
PDB None

Name CHEMBL1092023
Affinity Active
Assay description Binding affinity to human beta-casein at 1 mM by 1H-STD NMR analysis
-- Direct single protein target assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22889313
Doc title Toward rational fragment-based lead design without 3D structures.
PDB None

Name CHEMBL1334062
Affinity IC50 = 0.3uM
Assay description Inhibition of human TCF4/ flag epitope-tagged beta-catenin (unknown origin) interaction transfected in human HCT116 cells after 24 hrs by beta-galactosidase/luciferase reporter gene assay
-- Direct protein complex subunits assigned: TF7L2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24060489
Doc title Investigation of 3-aryl-pyrimido[5,4-e][1,2,4]triazine-5,7-diones as small molecule antagonists of beta-catenin/TCF transcription.
PDB None

Name CHEMBL1334062
Affinity IC50 = 0.3uM
Assay description Inhibition of human TCF4/ flag epitope-tagged beta-catenin (unknown origin) interaction transfected in human HCT116 cells after 24 hrs by beta-galactosidase/luciferase reporter gene assay
-- Direct protein complex subunits assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24060489
Doc title Investigation of 3-aryl-pyrimido[5,4-e][1,2,4]triazine-5,7-diones as small molecule antagonists of beta-catenin/TCF transcription.
PDB None

Name CHEMBL1628233
Affinity Active
Assay description Binding affinity to human beta-casein at 1 mM by 1H-STD NMR analysis
-- Direct single protein target assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22889313
Doc title Toward rational fragment-based lead design without 3D structures.
PDB None

Name CHEMBL179197
Affinity IC50 < 10uM
Assay description Inhibition of GST-tagged Tcf4 binding to human beta-catenin after 2 hrs by ELISA
-- Direct single protein target assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23566764
Doc title Direct targeting of beta-catenin: Inhibition of protein-protein interactions for the inactivation of Wnt signaling.
PDB None

Name CHEMBL2172378
Affinity Active
Assay description Binding affinity to human beta-casein at 0.5 mM by 1H-STD NMR analysis
-- Direct single protein target assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22889313
Doc title Toward rational fragment-based lead design without 3D structures.
PDB None

Name CHEMBL223231
Affinity Not Active
Assay description Activity against beta casein/Tcf4 complex by PPI assay
-- Direct single protein target assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/17455979
Doc title Additional scalarane sesterterpenes from the sponge Phyllospongia papyracea.
PDB None

Name CHEMBL223898
Affinity Not Active
Assay description Activity against beta casein/Tcf4 complex by PPI assay
-- Direct single protein target assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/17455979
Doc title Additional scalarane sesterterpenes from the sponge Phyllospongia papyracea.
PDB None

Name CHEMBL224775
Affinity Not Active
Assay description Activity against beta casein/Tcf4 complex by PPI assay
-- Direct single protein target assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/17455979
Doc title Additional scalarane sesterterpenes from the sponge Phyllospongia papyracea.
PDB None

Name CHEMBL224776
Affinity Not Active
Assay description Activity against beta casein/Tcf4 complex by PPI assay
-- Direct single protein target assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/17455979
Doc title Additional scalarane sesterterpenes from the sponge Phyllospongia papyracea.
PDB None

Name CHEMBL227444
Affinity Not Active
Assay description Activity against beta casein/Tcf4 complex by PPI assay
-- Direct single protein target assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/17455979
Doc title Additional scalarane sesterterpenes from the sponge Phyllospongia papyracea.
PDB None

Name CHEMBL227636
Affinity Not Active
Assay description Activity against beta casein/Tcf4 complex by PPI assay
-- Direct single protein target assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/17455979
Doc title Additional scalarane sesterterpenes from the sponge Phyllospongia papyracea.
PDB None

Name CHEMBL228073
Affinity Not Active
Assay description Activity against beta casein/Tcf4 complex by PPI assay
-- Direct single protein target assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/17455979
Doc title Additional scalarane sesterterpenes from the sponge Phyllospongia papyracea.
PDB None

Name CHEMBL2323033
Affinity IC50 < 10uM
Assay description Inhibition of GST-tagged Tcf4 binding to human beta-catenin after 2 hrs by ELISA
-- Direct single protein target assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23566764
Doc title Direct targeting of beta-catenin: Inhibition of protein-protein interactions for the inactivation of Wnt signaling.
PDB None

Name CHEMBL2397074
Affinity IC50 < 10uM
Assay description Inhibition of GST-tagged Tcf4 binding to human beta-catenin after 2 hrs by ELISA
-- Direct single protein target assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23566764
Doc title Direct targeting of beta-catenin: Inhibition of protein-protein interactions for the inactivation of Wnt signaling.
PDB None

Name CHEMBL2397075
Affinity IC50 < 10uM
Assay description Inhibition of GST-tagged Tcf4 binding to human beta-catenin after 2 hrs by ELISA
-- Direct single protein target assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23566764
Doc title Direct targeting of beta-catenin: Inhibition of protein-protein interactions for the inactivation of Wnt signaling.
PDB None

Name CHEMBL254381
Affinity Kd = 450nM
Assay description Inhibition of Tcf4 binding to beta-catenin (unknown origin)
-- Direct single protein target assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23566764
Doc title Direct targeting of beta-catenin: Inhibition of protein-protein interactions for the inactivation of Wnt signaling.
PDB None

Name CHEMBL254381
Affinity Activity = 0.45uM
Assay description Binding affinity to beta-casein
-- Homologous single protein target assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/18075579
Doc title Reaching for high-hanging fruit in drug discovery at protein-protein interfaces.
PDB None

Name CHEMBL460433
Affinity IC50 < 10uM
Assay description Inhibition of GST-tagged Tcf4 binding to human beta-catenin after 2 hrs by ELISA
-- Direct single protein target assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23566764
Doc title Direct targeting of beta-catenin: Inhibition of protein-protein interactions for the inactivation of Wnt signaling.
PDB None

Name CHEMBL578512
Affinity IC50 < 10uM
Assay description Inhibition of GST-tagged Tcf4 binding to human beta-catenin after 2 hrs by ELISA
-- Direct single protein target assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23566764
Doc title Direct targeting of beta-catenin: Inhibition of protein-protein interactions for the inactivation of Wnt signaling.
PDB None

Name CHEMBL91638
Affinity Active
Assay description Increase in axin1-beta-catenin interaction in human SW480 cells at 5 to 10 uM after 24 hrs by immunoprecipitation
-- Direct protein complex subunits assigned: CTNB1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24018191
Doc title Anti-proliferative activity of hydnocarpin, a natural lignan, is associated with the suppression of Wnt/beta-catenin signaling pathway in colon cancer cells.
PDB None

30 PDB entries for Beta-catenin and its protein partners


1DOW
Structure of the dimerization and beta-catenin-binding region of alpha-catenin.

Entity type Code Description
polypeptide(L) P26231 ALPHA-CATENIN
polypeptide(L) Q02248 BETA-CATENIN

1G3J
Crystal structure of a beta-catenin/Tcf complex.

Entity type Code Description
polypeptide(L) P35222 BETA-CATENIN ARMADILLO REPEAT REGION
polypeptide(L) P70062 TCF3-CBD (CATENIN BINDING DOMAIN)

1I7W
The structure of the beta-catenin/E-cadherin complex and the molecular basis of diverse ligand recognition by beta-catenin.

Entity type Code Description
polypeptide(L) Q02248 BETA-CATENIN
polypeptide(L) P09803 EPITHELIAL-CADHERIN

1I7X
The structure of the beta-catenin/E-cadherin complex and the molecular basis of diverse ligand recognition by beta-catenin.

Entity type Code Description
polypeptide(L) Q02248 BETA-CATENIN
polypeptide(L) P09803 EPITHELIAL-CADHERIN

1JDH
Tcf4 can specifically recognize beta-catenin using alternative conformations.

Entity type Code Description
polypeptide(L) P35222 BETA-CATENIN
polypeptide(L) Q9NQB0 hTcf-4

1JPP
Molecular mechanisms of beta-catenin recognition by adenomatous polyposis coli revealed by the structure of an APC-beta-catenin complex.

Entity type Code Description
polypeptide(L) Q02248 BETA-CATENIN
polypeptide(L) P25054 ADENOMATOUS POLYPOSIS COLI PROTEIN

1JPW
Structure of a human Tcf4-beta-catenin complex.

Entity type Code Description
polypeptide(L) P35222 BETA-CATENIN
polypeptide(L) Q9NQB0 transcription factor 7-like 2

1LUJ
The crystal structure of the beta-catenin/ICAT complex reveals the inhibitory mechanism of ICAT.

Entity type Code Description
polypeptide(L) P35222 Beta-catenin
polypeptide(L) Q9NSA3 beta-catenin-interacting protein ICAT

1M1E
ICAT inhibits Beta-catenin binding to Tcf/Lef-family transcription factors and the general coactivator p300 using independent structural modules.

Entity type Code Description
polypeptide(L) Q02248 Beta-catenin
polypeptide(L) Q9NSA3 ICAT

1P22
Structure of a beta-TrCP1-Skp1-beta-Catenin complex: destruction motif binding and lysine specificity of the SCFbeta-TrCP1 ubiquitin ligase

Entity type Code Description
polypeptide(L) Q9Y297 F-box/WD-repeat protein 1A
polypeptide(L) P63208 Skp1
polypeptide(L) P35222 Beta-catenin

1QZ7
Crystal structure of a beta-catenin/Axin complex suggests a mechanism for the {beta}-catenin destruction complex

Entity type Code Description
polypeptide(L) P35222 Beta-catenin
polypeptide(L) Q9YGY0 Axin

1T08
Mechanism of phosphorylation-dependent binding of APC to beta-catenin and its role in beta-catenin degradation

Entity type Code Description
polypeptide(L) P35222 Beta-catenin
polypeptide(L) Q9NSA3 Beta-catenin-interacting protein 1
polypeptide(L) P25054 Adenomatous polyposis coli protein

1TH1
Crystal Structure of a beta-Catenin/APC Complex Reveals a Critical Role for APC Phosphorylation in APC Function.

Entity type Code Description
polypeptide(L) P35222 Beta-catenin
polypeptide(L) P25054 Adenomatous polyposis coli protein

1V18
Mechanism of Phosphorylation-Dependent Binding of Apc to Beta-Catenin and its Role in Beta-Catenin Degradation

Entity type Code Description
polypeptide(L) Q02248 BETA-CATENIN
polypeptide(L) P25054 ADENOMATOUS POLYPOSIS COLI

2BCT
Three-dimensional structure of the armadillo repeat region of beta-catenin.

Entity type Code Description
polypeptide(L) Q02248 BETA-CATENIN

2G57
STD and TRNOESY NMR studies for the epitope mapping of the phosphorylation motif of the oncogenic protein beta-catenin recognized by a selective monoclonal antibody

Entity type Code Description
polypeptide(L) P35222 Beta-catenin

2GL7
Crystal Structure of a beta-Catenin/BCL9/Tcf4 Complex.

Entity type Code Description
polypeptide(L) P35222 Beta-catenin
polypeptide(L) Q9NQB0 Transcription factor 7-like 2
polypeptide(L) O00512 B-cell lymphoma 9 protein

2Z6H
Crystal Structure of a Full-Length beta-Catenin

Entity type Code Description
polypeptide(L) P35222 Catenin beta-1

3BCT
Three-dimensional structure of the armadillo repeat region of beta-catenin.

Entity type Code Description
polypeptide(L) Q02248 BETA-CATENIN

3DIW
Structural Basis of beta-Catenin Recognition by Tax-interacting Protein-1

Entity type Code Description
polypeptide(L) Q9DBG9 Tax1-binding protein 3
polypeptide(L) P35222 decameric peptide form Catenin beta-1

3OUW
Biochemical and structural characterization of beta-catenin interactions with non-phosphorylated and CK2-phosphorylated Lef-1

Entity type Code Description
polypeptide(L) Q02248 Catenin beta-1
polypeptide(L) P27782 Lymphoid enhancer-binding factor 1

3OUX
Biochemical and structural characterization of beta-catenin interactions with non-phosphorylated and CK2-phosphorylated Lef-1

Entity type Code Description
polypeptide(L) Q02248 Catenin beta-1
polypeptide(L) P27782 Lymphoid enhancer-binding factor 1

3SL9
An intrinsically labile alpha-helix abutting the BCL9-binding site of beta-catenin is required for its inhibition by carnosic acid.

Entity type Code Description
polypeptide(L) P35222 Catenin beta-1
polypeptide(L) O00512 B-cell CLL/lymphoma 9 protein
Ligand PEG DI(HYDROXYETHYL)ETHER
Ligand IMD IMIDAZOLE

3SLA
An intrinsically labile alpha-helix abutting the BCL9-binding site of beta-catenin is required for its inhibition by carnosic acid.

Entity type Code Description
polypeptide(L) P35222 Catenin beta-1

3TX7
Structural basis of coactivation of liver receptor homolog-1 by beta-catenin.

Entity type Code Description
polypeptide(L) P35222 Catenin beta-1
polypeptide(L) O00482 Nuclear receptor subfamily 5 group A member 2
Ligand P6L (2S)-3-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-2-[(6E)-HEXADEC-6-ENOYLOXY]PROPYL (8E)-OCTADEC-8-ENOATE

4DJS
Inhibition of oncogenic Wnt signaling through direct targeting of beta-catenin.

Entity type Code Description
polypeptide(L) P35222 Catenin beta-1
polypeptide(L) 4DJS stapled peptide RRWPQ(MK8)ILD(MK8)HVRRVWR

4EV8
Crystal structure of mouse catenin beta-59 in 2.4M urea.

Entity type Code Description
polypeptide(L) Q02248 Catenin beta-1

4EVA
Crystal Structure of Mouse Catenin beta-59 in 5.6M urea

Entity type Code Description
polypeptide(L) Q02248 Catenin beta-1

4EVP
Crystal Structure of Mouse Catenin beta-59 in 7.2M urea

Entity type Code Description
polypeptide(L) Q02248 Catenin beta-1

4EVT
Crystal Structure of Mouse Catenin beta-59 in 8.3M urea

Entity type Code Description
polypeptide(L) Q02248 Catenin beta-1