CD80 (B7-1)

T-lymphocyte activation antigen cd80 (activation b7-1 antigen)

Reviewed UniProt entries for CD80 (B7-1) and its protein partners


UniProt code UniProt Name Protein Name Gene Name Organism Length
P33681 CD80_HUMAN T-lymphocyte activation antigen CD80 (Activation B7-1 antigen) (BB1) (CTLA-4 counter-receptor B7.1) (B7) (CD antigen CD80) CD80 CD28LG CD28LG1 LAB7 Homo sapiens (Human) 288
Q00609 CD80_MOUSE T-lymphocyte activation antigen CD80 (Activation B7-1 antigen) (B7) (CD antigen CD80) Cd80 B7 Mus musculus (Mouse) 306
P42070 CD80_RABIT T-lymphocyte activation antigen CD80 (Activation B7-1 antigen) (CD antigen CD80) CD80 Oryctolagus cuniculus (Rabbit) 299
Q9XSI1 CTLA4_CANFA Cytotoxic T-lymphocyte protein 4 (Cytotoxic T-lymphocyte-associated antigen 4) (CTLA-4) (CD antigen CD152) CTLA4 Canis familiaris (Dog) (Canis lupus familiaris) 223
P16410 CTLA4_HUMAN Cytotoxic T-lymphocyte protein 4 (Cytotoxic T-lymphocyte-associated antigen 4) (CTLA-4) (CD antigen CD152) CTLA4 CD152 Homo sapiens (Human) 223
P09793 CTLA4_MOUSE Cytotoxic T-lymphocyte protein 4 (Cytotoxic T-lymphocyte-associated antigen 4) (CTLA-4) (CD antigen CD152) Ctla4 Cd152 Mus musculus (Mouse) 223
Q9MYX7 CTLA4_PIG Cytotoxic T-lymphocyte protein 4 (Cytotoxic T-lymphocyte-associated antigen 4) (CTLA-4) (CD antigen CD152) CTLA4 Sus scrofa (Pig) 223
P42072 CTLA4_RABIT Cytotoxic T-lymphocyte protein 4 (Cytotoxic T-lymphocyte-associated antigen 4) (CTLA-4) (CD antigen CD152) CTLA4 Oryctolagus cuniculus (Rabbit) 223
Q28071 CD28_BOVIN T-cell-specific surface glycoprotein CD28 (CD antigen CD28) CD28 Bos taurus (Bovine) 219
P31043 CD28_CHICK T-cell-specific surface glycoprotein CD28 homolog (CHT28) CD28 Gallus gallus (Chicken) 221
O02757 CD28_FELCA T-cell-specific surface glycoprotein CD28 (CD antigen CD28) CD28 Felis catus (Cat) (Felis silvestris catus) 221
P31041 CD28_MOUSE T-cell-specific surface glycoprotein CD28 (CD antigen CD28) Cd28 Mus musculus (Mouse) 218
P42069 CD28_RABIT T-cell-specific surface glycoprotein CD28 (CD antigen CD28) CD28 Oryctolagus cuniculus (Rabbit) 221
P31042 CD28_RAT T-cell-specific surface glycoprotein CD28 (CD antigen CD28) Cd28 Rattus norvegicus (Rat) 218
P10747 CD28_HUMAN T-cell-specific surface glycoprotein CD28 (TP44) (CD antigen CD28) CD28 Homo sapiens (Human) 220

Small molecules tested in CD80 (B7-1) binding assays:

4 datapoints for 4 distinct small molecules


Name JBS2007_1
Affinity Kd (BIACORE) 3uM
Assay description Surface Plasmon Resonance. CD80 inmobilised into and ligand added. Changes in the refractive index account for binding
-- Direct single protein target assigned: CD80_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1177/1087057107300464
Doc title Discovery of selective small-molecule CD80 inhibitors.
PDB None

Name JBS2007_6
Affinity Kd (BIACORE) 590nM
Assay description Surface Plasmon Resonance. CD80 inmobilised into and ligand added. Changes in the refractive index account for binding
-- Direct single protein target assigned: CD80_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1177/1087057107300464
Doc title Discovery of selective small-molecule CD80 inhibitors.
PDB None

Name BOMD11_6q
Affinity IC50 (ELISA) 4nM
Assay description inmobilised CD28; addind CD80 with 30 minpre-incubated cmps
-- Direct single protein target assigned: CD80_HUMAN (Expert curation) --
DOI http:/dx.doi.org/10.1016/S0968-0896(03)00183-4
Doc title Structure-activity studies of a series of dipyrazolo[3,4-b:3,4-d]pyridin-3-ones binding to the immune regulatory protein B7.1.
PDB None

Name JBC277_2
Affinity IC50 (ELISA) 30nM
Assay description inmobilised CD28; addind CD80 with 30 minpre-incubated cmps
-- Direct single protein target assigned: CD80_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1074/jbc.M110162200
Doc title Small molecule ligands define a binding site on the immune regulatory protein B7.1.
PDB None

10 PDB entries for CD80 (B7-1) and its protein partners


1AH1
Solution structure of human CTLA-4 and delineation of a CD80/CD86 binding site conserved in CD28.

Entity type Code Description
polypeptide(L) P16410 CTLA-4
Ligand None SUGAR (4-MER)
Ligand None SUGAR (4-MER)

1DQT
Structure of murine CTLA-4 and its role in modulating T cell responsiveness.

Entity type Code Description
polypeptide(L) P09793 CYTOTOXIC T LYMPHOCYTE ASSOCIATED ANTIGEN 4

1DR9
Structure and dimerization of a soluble form of B7-1.

Entity type Code Description
polypeptide(L) P33681 T LYMPHOCYTE ACTIVATION ANTIGEN
Ligand NAG SUGAR (N-ACETYL-D-GLUCOSAMINE)

1H6E
Study of the Interaction of the Medium Chain Mu2 Subunit of the Clathrin-Associated Adapter Protein Complex 2 with Cytotoxic T-Lymphocyte Antigen 4 and Cd28

Entity type Code Description
polypeptide(L) 1H6E CLATHRIN COAT ASSEMBLY PROTEIN AP50
polypeptide(L) P20172 CLATHRIN COAT ASSEMBLY PROTEIN AP50
polypeptide(L) P16410 CYTOTOXIC T-LYMPHOCYTE PROTEIN 4

1I85
Structural basis for co-stimulation by the human CTLA-4/B7-2 complex.

Entity type Code Description
polypeptide(L) P42081 T LYMPHOCYTE ACTIVATION ANTIGEN CD86
polypeptide(L) P16410 CYTOTOXIC T-LYMPHOCYTE-ASSOCIATED PROTEIN 4

1I8L
Crystal structure of the B7-1/CTLA-4 complex that inhibits human immune responses.

Entity type Code Description
polypeptide(L) P33681 T LYMPHOCYTE ACTIVATION ANTIGEN CD80
polypeptide(L) P16410 CYTOTOXIC T-LYMPHOCYTE PROTEIN 4
Ligand NAG SUGAR (N-ACETYL-D-GLUCOSAMINE)
Ligand MAN SUGAR (ALPHA-D-MANNOSE)

1YJD
Crystal structure of a soluble CD28-Fab complex

Entity type Code Description
polypeptide(L) 1YJD Fab fragment of 5.11A1 antibody light chain
polypeptide(L) 1YJD Fab fragment of 5.11A1 antibody heavy chain
polypeptide(L) P10747 T-cell-specific surface glycoprotein CD28
Ligand NAG SUGAR (N-ACETYL-D-GLUCOSAMINE)

2X44
Domain Metastability: A Molecular Basis for Immunoglobulin Deposition?

Entity type Code Description
polypeptide(L) 2X44 CYTOTOXIC T-LYMPHOCYTE PROTEIN 4
polypeptide(L) P16410 CYTOTOXIC T-LYMPHOCYTE PROTEIN 4

3BX7
High affinity molecular recognition and functional blockade of CTLA-4 by an engineered human lipocalin

Entity type Code Description
polypeptide(L) P16410 CYTOTOXIC T-LYMPHOCYTE-ASSOCIATED ANTIGEN 4
polypeptide(L) 3BX7 ENGINEERED HUMAN LIPOCALIN 2

3OSK
Rigid-body ligand recognition drives Cytotoxic T-lymphocyte Antigen 4 (CTLA-4) receptor triggering

Entity type Code Description
polypeptide(L) P16410 Cytotoxic T-lymphocyte protein 4
Ligand NAG SUGAR (N-ACETYL-D-GLUCOSAMINE)