Clathrin

Clathrin heavy chain 1

Reviewed UniProt entries for Clathrin and its protein partners


UniProt code UniProt Name Protein Name Gene Name Organism Length
Q00610 CLH1_HUMAN Clathrin heavy chain 1 (Clathrin heavy chain on chromosome 17) (CLH-17) CLTC CLH17 CLTCL2 KIAA0034 Homo sapiens (Human) 1675
P49951 CLHC1_BOVIN Clathrin heavy chain 1 CLTC Bos taurus (Bovine) 1675
Q68FD5 CLH_MOUSE Clathrin heavy chain 1 Cltc Mus musculus (Mouse) 1675
P11442 CLH_RAT Clathrin heavy chain 1 Cltc Rattus norvegicus (Rat) 1675
P50478 AMPH_CHICK Amphiphysin AMPH Gallus gallus (Chicken) 682
P49418 AMPH_HUMAN Amphiphysin AMPH AMPH1 Homo sapiens (Human) 695
Q7TQF7 AMPH_MOUSE Amphiphysin Amph Amph1 Mus musculus (Mouse) 686
O08838 AMPH_RAT Amphiphysin Amph Amph1 Rattus norvegicus (Rat) 683

Small molecules tested in Clathrin binding assays:

33 datapoints for 33 distinct small molecules


Name Pitstop_1
Affinity IC50 ELISA (ClathrinTD/amphiphysin) 18uM IC50 ELISA (ClathrinTD/amphiphysin)
Assay description ELISA-based screen for small molecules that can interfere with clathrin TD association of the B/C domain of amphiphysin; an endocytic protein harboring a clathrin box motif
-- Direct single protein target assigned: CLH1_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1016/j.cell.2011.06.025
Doc title Role of the clathrin terminal domain in regulating coated pit dynamics revealed by small molecule inhibition.
PDB 2XZG - (VH1)

Name Pitstop_2
Affinity IC50 ELISA (ClathrinTD/amphiphysin) 12uM IC50 ELISA (ClathrinTD/amphiphysin)
Assay description ELISA-based screen for small molecules that can interfere with clathrin TD association of the B/C domain of amphiphysin; an endocytic protein harboring a clathrin box motif
-- Direct single protein target assigned: CLH1_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1016/j.cell.2011.06.025
Doc title Role of the clathrin terminal domain in regulating coated pit dynamics revealed by small molecule inhibition.
PDB 2XZH - (VH2)

Name CHEMBL3110158
Affinity IC50 = 100uM
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110159
Affinity IC50 = 120uM
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110160
Affinity IC50 = 100uM
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110161
Affinity IC50 = 80uM
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110162
Affinity IC50 = 100uM
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110163
Affinity Not Active
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) at 100 uM after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110164
Affinity Not Active
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) at 100 uM after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110165
Affinity Not Active
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) at 100 uM after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110166
Affinity Not Active
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) at 100 uM after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110167
Affinity Not Active
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) at 100 uM after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110168
Affinity Not Active
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) at 100 uM after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110169
Affinity Not Active
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) at 100 uM after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110170
Affinity Not Active
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) at 100 uM after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110171
Affinity Not Active
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) at 100 uM after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110172
Affinity Not Active
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) at 100 uM after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110173
Affinity Not Active
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) at 100 uM after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110174
Affinity IC50 = 18uM
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110175
Affinity Not Active
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) at 100 uM after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110176
Affinity Not Active
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) at 100 uM after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110177
Affinity IC50 = 100uM
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110178
Affinity IC50 = 10uM
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110179
Affinity IC50 = 15uM
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110180
Affinity IC50 = 10uM
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110181
Affinity IC50 = 6.9uM
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110182
Affinity IC50 = 22uM
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110183
Affinity IC50 = 16uM
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110184
Affinity IC50 = 15uM
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110185
Affinity IC50 = 100uM
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110186
Affinity IC50 = 100uM
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110187
Affinity Not Active
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) at 100 uM after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

Name CHEMBL3110188
Affinity IC50 = 12uM
Assay description Inhibition of recombinant GST-tagged clathrin heavy chain (unknown origin) after 1 hr by ELISA
-- Direct single protein target assigned: CLH1_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24299503
Doc title
PDB None

18 PDB entries for Clathrin and its protein partners


1B89
Clathrin self-assembly is mediated by a tandemly repeated superhelix.

Entity type Code Description
polypeptide(L) P49951 PROTEIN (CLATHRIN HEAVY CHAIN)

1BPO
Atomic structure of clathrin: a beta propeller terminal domain joins an alpha zigzag linker.

Entity type Code Description
polypeptide(L) P11442 PROTEIN (CLATHRIN)

1C9I
Peptide-in-groove interactions link target proteins to the beta-propeller of clathrin.

Entity type Code Description
polypeptide(L) P11442 CLATHRIN
polypeptide(L) 1C9I B-ADAPTIN 3

1C9L
Peptide-in-groove interactions link target proteins to the beta-propeller of clathrin.

Entity type Code Description
polypeptide(L) P11442 CLATHRIN
polypeptide(L) 1C9L B-ADAPTIN 3

1KY7
Accessory protein recruitment motifs in clathrin-mediated endocytosis.

Entity type Code Description
polypeptide(L) P17427 ALPHA-ADAPTIN C
polypeptide(L) P49418 AMPHIPHYSIN

1UTC
Two Distinct Interaction Motifs in Amphiphysin Bind Two Independent Sites on the Clathrin Terminal Domain Beta-Propeller

Entity type Code Description
polypeptide(L) P49951 CLATHRIN HEAVY CHAIN
polypeptide(L) P49418 AMPHIPHYSIN

1XI4
Molecular model for a complete clathrin lattice from electron cryomicroscopy

Entity type Code Description
polypeptide(L) P49951 Clathrin heavy chain
polypeptide(L) P04973 Clathrin light chain A

1XI5
Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating

Entity type Code Description
polypeptide(L) P49951 Clathrin heavy chain
polypeptide(L) Q27974 Auxilin J-domain

2VJ0
Solitary and Repetitive Binding Motifs for the Ap2 Complex {Alpha}-Appendage in Amphiphysin and Other Accessory Proteins.

Entity type Code Description
polypeptide(L) 2VJ0 AP-2 COMPLEX SUBUNIT ALPHA-2
polypeptide(L) P17427 AP-2 COMPLEX SUBUNIT ALPHA-2
polypeptide(L) O43426 SYNAPTOJANIN-1
polypeptide(L) O08838 AMPHIPHYSIN
Ligand BEN BENZAMIDINE
Ligand DTD DITHIANE DIOL

2XZG
Role of the Clathrin Terminal Domain in Regulating Coated Pit Dynamics Revealed by Small Molecule Inhibition.

Entity type Code Description
polypeptide(L) Q00610 CLATHRIN HEAVY CHAIN 1
Ligand PEG DI(HYDROXYETHYL)ETHER
Ligand VH1 2-(4-AMINOBENZYL)-1,3-DIOXO-2,3-DIHYDRO-1H-BENZO[DE]ISOQUINOLINE-5-SULFONATE

3GC3
Structure of an arrestin2-clathrin complex reveals a novel clathrin binding domain that modulates receptor trafficking.

Entity type Code Description
polypeptide(L) P17870 Beta-arrestin-1
polypeptide(L) P49951 Clathrin heavy chain 1

3GD1
Structure of an arrestin2-clathrin complex reveals a novel clathrin binding domain that modulates receptor trafficking

Entity type Code Description
polypeptide(L) P17870 Beta-arrestin-1
polypeptide(L) P49951 Clathrin heavy chain 1
polypeptide(L) None clathrin

3IYV
Molecular model for a complete clathrin lattice from electron cryomicroscopy.

Entity type Code Description
polypeptide(L) P49951 Clathrin heavy chain
polypeptide(L) P04973 Clathrin light chain A

3LVG
Conformation switching of clathrin light chain regulates clathrin lattice assembly.

Entity type Code Description
polypeptide(L) P49951 Clathrin heavy chain 1
polypeptide(L) 3LVG Clathrin light chain B
polypeptide(L) P04975 Clathrin light chain B

3LVH
Conformation switching of clathrin light chain regulates clathrin lattice assembly.

Entity type Code Description
polypeptide(L) P49951 Clathrin heavy chain 1
polypeptide(L) P04975 Clathrin light chain B
polypeptide(L) 3LVH Clathrin light chain B

3QIL
Nuclear localization of clathrin involves a labile helix outside the trimerization domain.

Entity type Code Description
polypeptide(L) P49951 Clathrin heavy chain 1

4ATM
Crystal Structure of the Bar Domain of Human Amphiphysin, Isoform 1

Entity type Code Description
polypeptide(L) P49418 AMPHIPHYSIN

4G55
Role of the clathrin terminal domain in regulating coated pit dynamics revealed by small molecule inhibition.

Entity type Code Description
polypeptide(L) Q00610 Clathrin heavy chain 1
Ligand PEG DI(HYDROXYETHYL)ETHER
Ligand VH2 N-[5-[(4-BROMOPHENYL)METHYL]-4-HYDROXY-1,3-THIAZOL-2-YL]NAPHTHALENE-1-SULFONAMIDE