MDM2

E3 ubiquitin-protein ligase mdm2 (double minute 2 protein)

Reviewed UniProt entries for MDM2 and its protein partners


UniProt code UniProt Name Protein Name Gene Name Organism Length
P56950 MDM2_CANFA E3 ubiquitin-protein ligase Mdm2 (EC 6.3.2.-) (Double minute 2 protein) (Cdm2) (p53-binding protein Mdm2) MDM2 Canis familiaris (Dog) (Canis lupus familiaris) 487
O42354 MDM2_DANRE E3 ubiquitin-protein ligase Mdm2 (EC 6.3.2.-) (Double minute 2 protein) (p53-binding protein Mdm2) mdm2 Danio rerio (Zebrafish) (Brachydanio rerio) 445
Q7YRZ8 MDM2_FELCA E3 ubiquitin-protein ligase Mdm2 (EC 6.3.2.-) (Double minute 2 protein) (p53-binding protein Mdm2) MDM2 Felis catus (Cat) (Felis silvestris catus) 491
P56951 MDM2_HORSE E3 ubiquitin-protein ligase Mdm2 (EC 6.3.2.-) (Double minute 2 protein) (Edm2) (p53-binding protein Mdm2) MDM2 Equus caballus (Horse) 491
Q00987 MDM2_HUMAN E3 ubiquitin-protein ligase Mdm2 (EC 6.3.2.-) (Double minute 2 protein) (Hdm2) (Oncoprotein Mdm2) (p53-binding protein Mdm2) MDM2 Homo sapiens (Human) 491
Q60524 MDM2_MESAU E3 ubiquitin-protein ligase Mdm2 (EC 6.3.2.-) (Double minute 2 protein) (Oncoprotein Mdm2) (p53-binding protein Mdm2) (Fragment) MDM2 Mesocricetus auratus (Golden hamster) 466
P23804 MDM2_MOUSE E3 ubiquitin-protein ligase Mdm2 (EC 6.3.2.-) (Double minute 2 protein) (Oncoprotein Mdm2) (p53-binding protein Mdm2) Mdm2 Mus musculus (Mouse) 489
P56273 MDM2_XENLA E3 ubiquitin-protein ligase Mdm2 (EC 6.3.2.-) (Double minute 2 protein) (Xdm2) (p53-binding protein Mdm2) mdm2 Xenopus laevis (African clawed frog) 473

Small molecules tested in MDM2 binding assays:

600 datapoints for 416 distinct small molecules


Name Nutlin_16
Affinity IC50 SPR 140nM
Assay description p53 immobilized to biosensor; MDM2 + lig add; inhibition of MDM2 binding leads to decreased biosensor response
-- Direct single protein target assigned: MDM2_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1002/bip.20741
Doc title Targeting protein-protein interactions: lessons from p53/MDM2.
PDB 1RV1 - (IMZ)

Name Benzodiazep_18
Affinity IC50 FP 80nM
Assay description ThermoFluor Monitor melting point by flourences dyes to see unfolding of the protein
-- Direct single protein target assigned: MDM2_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1002/bip.20741
Doc title Targeting protein-protein interactions: lessons from p53/MDM2.
PDB 1T4E - (DIZ)

Name BetaHarpin_51
Affinity IC50 SPR 140nM
Assay description None
-- Direct single protein target assigned: MDM2_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1002/bip.20741
Doc title Targeting protein-protein interactions: lessons from p53/MDM2.
PDB 2AXI - (None)

Name Chalcone_9
Affinity IC50 ELISA 200uM
Assay description MDM2 inmobilized; add p53 + lig; wash; p53AB; p53AB-AB; enzyme to catalize flourescent prod from non-fluo- reagent; add of this reagent
-- Direct single protein target assigned: MDM2_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1002/bip.20741
Doc title Targeting protein-protein interactions: lessons from p53/MDM2.
PDB None

Name HexylitaconicAcid
Affinity IC50 ELISA 230uM
Assay description None
-- Direct single protein target assigned: MDM2_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1002/bip.20741
Doc title Targeting protein-protein interactions: lessons from p53/MDM2.
PDB None

Name Syc-7
Affinity weak; but cell active weak; but cell active
Assay description None
-- Direct single protein target assigned: MDM2_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1002/bip.20741
Doc title Targeting protein-protein interactions: lessons from p53/MDM2.
PDB None

Name Sulfonamide14
Affinity IC50 FP 32uM
Assay description None
-- Direct single protein target assigned: MDM2_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1002/bip.20741
Doc title Targeting protein-protein interactions: lessons from p53/MDM2.
PDB None

Name Nutlin_15
Affinity IC50 SPR 260nM
Assay description p53 immobilized to biosensor; MDM2 + lig add; inhibition of MDM2 binding leads to decreased biosensor response
-- Direct single protein target assigned: MDM2_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1002/bip.20741
Doc title Targeting protein-protein interactions: lessons from p53/MDM2.
PDB None

Name Nutlin_17
Affinity IC50 SPR 90nM
Assay description p53 immobilized to biosensor; MDM2 + lig add; inhibition of MDM2 binding leads to decreased biosensor response
-- Direct single protein target assigned: MDM2_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1002/bip.20741
Doc title Targeting protein-protein interactions: lessons from p53/MDM2.
PDB None

Name Benzodiazep_19
Affinity IC50 FP 708nM
Assay description Improved cell permeability
-- Direct single protein target assigned: MDM2_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1002/bip.20741
Doc title Targeting protein-protein interactions: lessons from p53/MDM2.
PDB None

Name Benzodiazep_20
Affinity IC50 FP 704nM
Assay description Improved cell permeability
-- Direct single protein target assigned: MDM2_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1002/bip.20741
Doc title Targeting protein-protein interactions: lessons from p53/MDM2.
PDB None

Name Spiro-oxiIndole_21
Affinity IC50 FP 8uM
Assay description None
-- Direct single protein target assigned: MDM2_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1002/bip.20741
Doc title Targeting protein-protein interactions: lessons from p53/MDM2.
PDB None

Name Spiro-oxiIndole_22
Affinity IC50 FP 86nM
Assay description improved cell act
-- Direct single protein target assigned: MDM2_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1002/bip.20741
Doc title Targeting protein-protein interactions: lessons from p53/MDM2.
PDB None

Name Isoindolinone_23
Affinity IC50 ELISA 16uM
Assay description None
-- Direct single protein target assigned: MDM2_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1002/bip.20741
Doc title Targeting protein-protein interactions: lessons from p53/MDM2.
PDB None

Name p-Oligobenzamides
Affinity Kd 12uM
Assay description although they made all analogues; the one with the right subs was much less active. No further discussion
-- Direct single protein target assigned: MDM2_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1002/bip.20741
Doc title Targeting protein-protein interactions: lessons from p53/MDM2.
PDB None

Name JBNMR30_1
Affinity IC50 160nM
Assay description Competition assay against p53 peptide
-- Direct single protein target assigned: MDM2_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1023/B:JNMR.0000048856.84603.9b
Doc title NMR structure of a complex between MDM2 and a small molecule inhibitor.
PDB 1TTV - (IMY)

Name WK23
Affinity Ki (FP) 916 nM
Assay description Fluorescence polorization assay
-- Direct single protein target assigned: MDM4_HUMAN (Expert curation) --
DOI http://www.landesbioscience.com/journals/cc/article/10956/
Doc title Structures of low molecular weight inhibitors bound to MDMX and MDM2 reveal new approaches for p53-MDMX/MDM2 antagonist drug discovery
PDB 3LBK - (K23)

Name MI-63_analog
Affinity Ki (FP) 36 nM
Assay description Fluorescence polorization assay
-- Direct single protein target assigned: MDM4_HUMAN (Expert curation) --
DOI http://www.landesbioscience.com/journals/cc/article/10956/
Doc title Structures of low molecular weight inhibitors bound to MDMX and MDM2 reveal new approaches for p53-MDMX/MDM2 antagonist drug discovery
PDB 3LBL - (MI6)

Name CHEMBL1094278
Affinity Not Active
Assay description Inhibition of recombinant RING-E3 ligase Mdm2 autoubiquitination at 5 to 50 uM after 30 mins by Western blotting in presence of human recombinant E2 UbcH5b
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20222671
Doc title Exploring the structural requirements for inhibition of the ubiquitin E3 ligase breast cancer associated protein 2 (BCA2) as a treatment for breast cancer.
PDB None

Name CHEMBL1097571
Affinity Not Active
Assay description Inhibition of recombinant RING-E3 ligase Mdm2 autoubiquitination at 5 to 50 uM after 30 mins by Western blotting in presence of human recombinant E2 UbcH5b
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20222671
Doc title Exploring the structural requirements for inhibition of the ubiquitin E3 ligase breast cancer associated protein 2 (BCA2) as a treatment for breast cancer.
PDB None

Name CHEMBL121516
Affinity Not Active
Assay description Inhibition of recombinant RING-E3 ligase Mdm2 autoubiquitination at 5 to 50 uM after 30 mins by Western blotting in presence of human recombinant E2 UbcH5b
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20222671
Doc title Exploring the structural requirements for inhibition of the ubiquitin E3 ligase breast cancer associated protein 2 (BCA2) as a treatment for breast cancer.
PDB None

Name CHEMBL1233798
Affinity Ki = 0.92uM
Assay description Binding affinity to MDM2 (unknown origin) expressed in Escherichia coli BL21(DE3) after 30 mins by fluorescence polarization assay in presence of P4 peptide
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22789708
Doc title Benzimidazole-2-one: a novel anchoring principle for antagonizing p53-Mdm2.
PDB 3LBK - (K23)

Name CHEMBL1234332
Affinity Ki = 0.003uM
Assay description Binding affinity to MDM2 (unknown origin) expressed in Escherichia coli BL21(DE3) after 30 mins by fluorescence polarization assay in presence of P4 peptide
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22789708
Doc title Benzimidazole-2-one: a novel anchoring principle for antagonizing p53-Mdm2.
PDB 3LBL - (MI6)

Name CHEMBL1256047
Affinity INH = 16%
Assay description Inhibition of GST-tagged p53 binding to Hdm2 at 560 uM by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20638853
Doc title Functional profiling of p53-binding sites in Hdm2 and Hdmx using a genetic selection system.
PDB None

Name CHEMBL1256056
Affinity Not Active
Assay description Inhibition of GST-tagged p53 binding to Hdm2 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20638853
Doc title Functional profiling of p53-binding sites in Hdm2 and Hdmx using a genetic selection system.
PDB None

Name CHEMBL1256057
Affinity Not Active
Assay description Inhibition of GST-tagged p53 binding to Hdm2 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20638853
Doc title Functional profiling of p53-binding sites in Hdm2 and Hdmx using a genetic selection system.
PDB None

Name CHEMBL1256058
Affinity Not Active
Assay description Inhibition of GST-tagged p53 binding to Hdm2 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20638853
Doc title Functional profiling of p53-binding sites in Hdm2 and Hdmx using a genetic selection system.
PDB None

Name CHEMBL1256059
Affinity Not Active
Assay description Inhibition of GST-tagged p53 binding to Hdm2 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20638853
Doc title Functional profiling of p53-binding sites in Hdm2 and Hdmx using a genetic selection system.
PDB None

Name CHEMBL1256060
Affinity Not Active
Assay description Inhibition of GST-tagged p53 binding to Hdm2 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20638853
Doc title Functional profiling of p53-binding sites in Hdm2 and Hdmx using a genetic selection system.
PDB None

Name CHEMBL1256061
Affinity Not Active
Assay description Inhibition of GST-tagged p53 binding to Hdm2 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20638853
Doc title Functional profiling of p53-binding sites in Hdm2 and Hdmx using a genetic selection system.
PDB None

Name CHEMBL1256062
Affinity Not Active
Assay description Inhibition of GST-tagged p53 binding to Hdm2 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20638853
Doc title Functional profiling of p53-binding sites in Hdm2 and Hdmx using a genetic selection system.
PDB None

Name CHEMBL1256064
Affinity Not Active
Assay description Inhibition of GST-tagged p53 binding to Hdm2 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20638853
Doc title Functional profiling of p53-binding sites in Hdm2 and Hdmx using a genetic selection system.
PDB None

Name CHEMBL1256067
Affinity Not Active
Assay description Inhibition of GST-tagged p53 binding to Hdm2 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20638853
Doc title Functional profiling of p53-binding sites in Hdm2 and Hdmx using a genetic selection system.
PDB None

Name CHEMBL1256068
Affinity Not Active
Assay description Inhibition of GST-tagged p53 binding to Hdm2 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20638853
Doc title Functional profiling of p53-binding sites in Hdm2 and Hdmx using a genetic selection system.
PDB None

Name CHEMBL1256069
Affinity Not Active
Assay description Inhibition of GST-tagged p53 binding to Hdm2 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20638853
Doc title Functional profiling of p53-binding sites in Hdm2 and Hdmx using a genetic selection system.
PDB None

Name CHEMBL1256070
Affinity Not Active
Assay description Inhibition of GST-tagged p53 binding to Hdm2 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20638853
Doc title Functional profiling of p53-binding sites in Hdm2 and Hdmx using a genetic selection system.
PDB None

Name CHEMBL1256071
Affinity Not Active
Assay description Inhibition of GST-tagged p53 binding to Hdm2 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20638853
Doc title Functional profiling of p53-binding sites in Hdm2 and Hdmx using a genetic selection system.
PDB None

Name CHEMBL1256073
Affinity Not Active
Assay description Inhibition of GST-tagged p53 binding to Hdm2 at 560 uM by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20638853
Doc title Functional profiling of p53-binding sites in Hdm2 and Hdmx using a genetic selection system.
PDB None

Name CHEMBL151233
Affinity Not Active
Assay description Inhibition of GST fused human Hdm2 in human RPE cells assessed as increase in p53 level at 1 to 10 uM after 8 hrs by immunoblotting
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/18977148
Doc title Discovery of new pyridoacridine alkaloids from Lissoclinum cf. badium that inhibit the ubiquitin ligase activity of Hdm2 and stabilize p53.
PDB None

Name CHEMBL151233
Affinity Not Active
Assay description Inhibition of GST fused human Hdm2 in human RPE cells at 1 to 10 uM after 8 hrs by immunoblotting
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/18977148
Doc title Discovery of new pyridoacridine alkaloids from Lissoclinum cf. badium that inhibit the ubiquitin ligase activity of Hdm2 and stabilize p53.
PDB None

Name CHEMBL151233
Affinity IC50 > 295uM
Assay description Inhibition of GST fused human Hdm2 assessed as polyubiquitylation by electrochemiluminescence assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/18977148
Doc title Discovery of new pyridoacridine alkaloids from Lissoclinum cf. badium that inhibit the ubiquitin ligase activity of Hdm2 and stabilize p53.
PDB None

Name CHEMBL1688136
Affinity IC50 = 69uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688137
Affinity IC50 = 74uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688138
Affinity IC50 > 20uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688139
Affinity IC50 = 4.2uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688140
Affinity IC50 = 4.5uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688141
Affinity IC50 > 20uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688142
Affinity IC50 = 15uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688143
Affinity IC50 = 6uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688144
Affinity IC50 = 87uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688145
Affinity IC50 = 9.5uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688146
Affinity IC50 = 2.4uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688147
Affinity IC50 = 3.1uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688148
Affinity IC50 = 54uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688149
Affinity IC50 = 7.2uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688150
Affinity IC50 = 6.2uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688151
Affinity IC50 = 6.2uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688152
Affinity IC50 = 47uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688153
Affinity IC50 > 100uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688154
Affinity IC50 > 20uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688155
Affinity IC50 > 100uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688156
Affinity IC50 > 100uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688157
Affinity IC50 > 100uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688158
Affinity IC50 > 100uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688159
Affinity IC50 = 11uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688160
Affinity IC50 > 100uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688161
Affinity IC50 = 88uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688162
Affinity IC50 > 20uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688163
Affinity IC50 > 20uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688164
Affinity IC50 > 200uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688165
Affinity IC50 > 200uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688166
Affinity IC50 > 200uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688167
Affinity IC50 > 200uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688168
Affinity IC50 > 200uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688169
Affinity IC50 > 20uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688170
Affinity IC50 = 3uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688251
Affinity IC50 = 0.7uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688252
Affinity IC50 = 0.4uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688253
Affinity IC50 = 2.3uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688254
Affinity IC50 = 4.7uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688255
Affinity IC50 = 1.5uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688256
Affinity IC50 = 1.4uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688257
Affinity IC50 = 1.9uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688258
Affinity IC50 = 0.4uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688259
Affinity IC50 = 0.45uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688260
Affinity IC50 = 0.36uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688261
Affinity IC50 = 3.5uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688262
Affinity IC50 = 0.9uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688263
Affinity IC50 = 2.5uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688264
Affinity IC50 = 3.2uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688265
Affinity IC50 = 1.4uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688266
Affinity IC50 = 0.57uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688267
Affinity IC50 = 0.98uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688268
Affinity Active
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p53 protein at 10 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688268
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p21 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688268
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of MDM2 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688268
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p53 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688268
Affinity IC50 = 0.58uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688268
Affinity Active
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p21 protein at 10 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688269
Affinity IC50 = 0.73uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688270
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p21 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688270
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of MDM2 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688270
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p53 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688270
Affinity IC50 = 0.38uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688271
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p21 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688271
Affinity IC50 = 0.66uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688271
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p53 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688271
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of MDM2 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688272
Affinity Active
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p53 protein at 10 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688272
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p21 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688272
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of MDM2 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688272
Affinity IC50 = 0.57uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688272
Affinity Active
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p21 protein at 10 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688272
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p53 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688273
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p53 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688273
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of MDM2 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688273
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p21 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688273
Affinity IC50 = 0.4uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688274
Affinity Active
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p53 protein at 10 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688274
Affinity IC50 = 0.23uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688274
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p53 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688274
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of MDM2 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688274
Affinity Active
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p21 protein at 10 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688274
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p21 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688275
Affinity IC50 = 0.39uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688276
Affinity IC50 = 0.31uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688277
Affinity IC50 = 1.8uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688278
Affinity IC50 = 2.6uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688279
Affinity IC50 = 2.3uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688280
Affinity IC50 = 1.2uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688281
Affinity IC50 = 1.5uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688282
Affinity IC50 = 2.3uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688283
Affinity IC50 = 2.8uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688284
Affinity IC50 = 8.8uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688285
Affinity IC50 = 8.9uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688286
Affinity IC50 = 49uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688287
Affinity IC50 = 47uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688288
Affinity IC50 = 214uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688289
Affinity IC50 = 175uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688290
Affinity IC50 > 20uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688291
Affinity IC50 > 20uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688292
Affinity IC50 > 50uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688293
Affinity IC50 = 17uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688294
Affinity IC50 > 100uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688295
Affinity IC50 = 252uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688296
Affinity IC50 = 29uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688297
Affinity IC50 = 11uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688298
Affinity IC50 > 200uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688299
Affinity IC50 > 200uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688300
Affinity IC50 = 33uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688301
Affinity IC50 > 20uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688302
Affinity IC50 > 50uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688303
Affinity Active
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p21 protein at 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688303
Affinity IC50 = 0.17uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688303
Affinity Active
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of MDM2 protein at 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688303
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p21 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688303
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of MDM2 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688303
Affinity Active
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p53 protein at 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688303
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p53 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688304
Affinity Not Active
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p53 protein at 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688304
Affinity Not Active
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p21 protein at 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688304
Affinity Not Active
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of MDM2 protein at 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688304
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of MDM2 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688304
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p21 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688304
Affinity IC50 = 1.3uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688304
Affinity Dose-dependent effect
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p53 protein at 1 to 20 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL1688305
Affinity IC50 = 16uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL178578
Affinity IC50 = 1.7uM
Assay description Binding affinity between MDM2 and p53 protein in fluorescence peptide assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/15780621
Doc title Structure-based design, synthesis, and biological evaluation of novel 1,4-diazepines as HDM2 antagonists.
PDB None

Name CHEMBL1818538
Affinity IC50 = 100nM
Assay description Antagonist activity at human GST-tagged MDM2 assessed as inhibition of binding to full length p53
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23541651
Doc title Inhibitors of the p53/hdm2 protein-protein interaction-path to the clinic.
PDB None

Name CHEMBL182051
Affinity IC50 = 30uM
Assay description Binding affinity between MDM2 and p53 protein in fluorescence peptide assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/15780621
Doc title Structure-based design, synthesis, and biological evaluation of novel 1,4-diazepines as HDM2 antagonists.
PDB None

Name CHEMBL1909836
Affinity Ki = 6.7uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL191334
Affinity IC50 = 0.061uM
Assay description Inhibition of Mdm2 -p53 protein interaction by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL191334
Affinity Active
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p21 protein at 1 to 10 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL191334
Affinity IC50 = 100nM
Assay description Antagonist activity at human GST-tagged MDM2 assessed as inhibition of binding to full length p53
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23541651
Doc title Inhibitors of the p53/hdm2 protein-protein interaction-path to the clinic.
PDB None

Name CHEMBL191334
Affinity IC50 = 1uM
Assay description Binding to HDM2 expressed in Escherichia coli BL21 (DE3) by fluorescence anisotropy competition method
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/19880322
Doc title N-acylpolyamine inhibitors of HDM2 and HDMX binding to p53.
PDB None

Name CHEMBL191334
Affinity Ki = 0.036uM
Assay description Displacement of PMDM6-F from human recombinant MDM2
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16759082
Doc title Structure-based design of spiro-oxindoles as potent, specific small-molecule inhibitors of the MDM2-p53 interaction.
PDB None

Name CHEMBL191334
Affinity Ki = 0.121uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL191334
Affinity IC50 = 600nM
Assay description Inhibition of human GST-tagged Mdm2 expressed in Escherichia coli harboring integrated p53-Mmd2 protein assessed as blockade of enzyme-p53 interaction by ELISA assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21277201
Doc title Synthesis of cell-permeable stapled peptide dual inhibitors of the p53-Mdm2/Mdmx interactions via photoinduced cycloaddition.
PDB None

Name CHEMBL191334
Affinity Dose-dependent effect
Assay description Inhibition of MDM2-p53 interaction in human SJSA1 cells assessed as accumulation of p21 after 24 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23786219
Doc title A potent small-molecule inhibitor of the MDM2-p53 interaction (MI-888) achieved complete and durable tumor regression in mice.
PDB None

Name CHEMBL191334
Affinity IC50 = 0.09uM
Assay description Inhibition of MDM2 (unknown origin) assessed as activation of p53
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23477941
Doc title Synthesis and evaluation of an imidazole derivative-fluorescein conjugate.
PDB None

Name CHEMBL191334
Affinity Dose-dependent effect
Assay description Inhibition of MDM2-p53 interaction in human SJSA1 cells assessed as p53 activation after 24 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23786219
Doc title A potent small-molecule inhibitor of the MDM2-p53 interaction (MI-888) achieved complete and durable tumor regression in mice.
PDB None

Name CHEMBL191334
Affinity IC50 = 0.09uM
Assay description Inhibition of MDM2 binding to p53
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL191334
Affinity Dose-dependent effect
Assay description Inhibition of MDM2-p53 interaction in human SJSA1 cells assessed as MDM2 accumulation after 24 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23786219
Doc title A potent small-molecule inhibitor of the MDM2-p53 interaction (MI-888) achieved complete and durable tumor regression in mice.
PDB None

Name CHEMBL191334
Affinity Kd = 0.7uM
Assay description Binding affinity to human wild type Mdm2 by isothermal titration calorimetry
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/18680271
Doc title NMR screening for lead compounds using tryptophan-mutated proteins.
PDB None

Name CHEMBL191334
Affinity Active
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of MDM2 protein at 1 to 10 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL191334
Affinity IC50 = 90nM
Assay description Inhibition of HDM2 binding to p53
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22227462
Doc title Chemical modulators working at pharmacological interface of target proteins.
PDB None

Name CHEMBL191334
Affinity Active
Assay description Inhibition of Mdm2 -p53 protein interaction in human SNJSA1 cells assessed as induction of p53 protein at 1 to 10 uM after 6 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/21314128
Doc title Isoindolinone inhibitors of the murine double minute 2 (MDM2)-p53 protein-protein interaction: structure-activity studies leading to improved potency.
PDB None

Name CHEMBL191334
Affinity IC50 = 0.089uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL191334
Affinity IC50 = 0.018uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL191334
Affinity IC50 = 90nM
Assay description Binding affinity to human HDM2 assessed as inhibition of HDM2-p53 interaction
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/18075579
Doc title Reaching for high-hanging fruit in drug discovery at protein-protein interfaces.
PDB None

Name CHEMBL191334
Affinity IC50 = 13.6uM
Assay description Binding affinity to MDM2
-- Homologous single protein target assigned: MDM2_HUMAN (Intermediate curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/18752944
Doc title Targeted intracellular protein degradation induced by a small molecule: En route to chemical proteomics.
PDB None

Name CHEMBL191334
Affinity Dose-dependent effect
Assay description Inhibition of MDM2-P53 interaction in human SJSA1 cells assessed as p53-mediated decrease in full length PARP level at 24 hrs by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23786219
Doc title A potent small-molecule inhibitor of the MDM2-p53 interaction (MI-888) achieved complete and durable tumor regression in mice.
PDB None

Name CHEMBL191334
Affinity Ki = 36nM
Assay description Binding affinity to MDM2
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16789731
Doc title Discovery of a nanomolar inhibitor of the human murine double minute 2 (MDM2)-p53 interaction through an integrated, virtual database screening strategy.
PDB None

Name CHEMBL199341
Affinity IC50 = 50ug ml-1
Assay description Inhibition of p53-HDM2 interaction
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16246554
Doc title Hexylitaconic acid: a new inhibitor of p53-HDM2 interaction isolated from a marine-derived fungus, Arthrinium sp.
PDB None

Name CHEMBL199687
Affinity Not Active
Assay description Inhibition of p53-HDM2 interaction at 50 ug/ml
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16246554
Doc title Hexylitaconic acid: a new inhibitor of p53-HDM2 interaction isolated from a marine-derived fungus, Arthrinium sp.
PDB None

Name CHEMBL2009640
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2059299
Affinity IC50 = 18.9uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB 2LZG - (13Q)

Name CHEMBL2059299
Affinity IC50 = 2.4uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB 2LZG - (13Q)

Name CHEMBL2059300
Affinity Kd = 1nM
Assay description Binding affinity to human MDM2 by SPR analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22524527
Doc title Structure-based design of novel inhibitors of the MDM2-p53 interaction.
PDB None

Name CHEMBL2059435
Affinity Kd = 0.4nM
Assay description Binding affinity to human MDM2 by surface plasmon resonance binding assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22624960
Doc title AM-8553: a novel MDM2 inhibitor with a promising outlook for potential clinical development.
PDB 4ERF - (0R3)

Name CHEMBL2059435
Affinity Kd = 0.4nM
Assay description Binding affinity to human MDM2 by SPR analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22524527
Doc title Structure-based design of novel inhibitors of the MDM2-p53 interaction.
PDB 4ERF - (0R3)

Name CHEMBL207013
Affinity IC50 > 12.5uM
Assay description Displacement of p53 mimicking fluorescent peptide from Hdm2 by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16630722
Doc title Enantiomerically pure 1,4-benzodiazepine-2,5-diones as Hdm2 antagonists.
PDB None

Name CHEMBL207202
Affinity IC50 = 2.39uM
Assay description Displacement of p53 mimicking fluorescent peptide from Hdm2 by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16630722
Doc title Enantiomerically pure 1,4-benzodiazepine-2,5-diones as Hdm2 antagonists.
PDB None

Name CHEMBL207255
Affinity IC50 = 0.85uM
Assay description Inhibition p53-HDM2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16600594
Doc title Enhanced pharmacokinetic properties of 1,4-benzodiazepine-2,5-dione antagonists of the HDM2-p53 protein-protein interaction through structure-based drug design.
PDB None

Name CHEMBL207332
Affinity IC50 = 0.25uM
Assay description Inhibition of p53-Hdm2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16647257
Doc title Novel 1,4-benzodiazepine-2,5-diones as Hdm2 antagonists with improved cellular activity.
PDB None

Name CHEMBL207364
Affinity IC50 = 0.787uM
Assay description Displacement of p53 mimicking fluorescent peptide from Hdm2 by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16630722
Doc title Enantiomerically pure 1,4-benzodiazepine-2,5-diones as Hdm2 antagonists.
PDB None

Name CHEMBL207505
Affinity IC50 = 13.3uM
Assay description Inhibition p53-HDM2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16600594
Doc title Enhanced pharmacokinetic properties of 1,4-benzodiazepine-2,5-dione antagonists of the HDM2-p53 protein-protein interaction through structure-based drug design.
PDB None

Name CHEMBL207541
Affinity IC50 = 5.4uM
Assay description Displacement of p53 mimicking fluorescent peptide from Hdm2 by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16630722
Doc title Enantiomerically pure 1,4-benzodiazepine-2,5-diones as Hdm2 antagonists.
PDB None

Name CHEMBL207845
Affinity IC50 = 1.04uM
Assay description Inhibition p53-HDM2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16600594
Doc title Enhanced pharmacokinetic properties of 1,4-benzodiazepine-2,5-dione antagonists of the HDM2-p53 protein-protein interaction through structure-based drug design.
PDB None

Name CHEMBL208007
Affinity IC50 = 2.15uM
Assay description Inhibition p53-HDM2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16600594
Doc title Enhanced pharmacokinetic properties of 1,4-benzodiazepine-2,5-dione antagonists of the HDM2-p53 protein-protein interaction through structure-based drug design.
PDB None

Name CHEMBL208020
Affinity IC50 = 4.35uM
Assay description Inhibition p53-HDM2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16600594
Doc title Enhanced pharmacokinetic properties of 1,4-benzodiazepine-2,5-dione antagonists of the HDM2-p53 protein-protein interaction through structure-based drug design.
PDB None

Name CHEMBL208554
Affinity IC50 = 0.81uM
Assay description Inhibition of p53-Hdm2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16647257
Doc title Novel 1,4-benzodiazepine-2,5-diones as Hdm2 antagonists with improved cellular activity.
PDB None

Name CHEMBL208562
Affinity IC50 = 0.546uM
Assay description Displacement of p53 mimicking fluorescent peptide from Hdm2 by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16630722
Doc title Enantiomerically pure 1,4-benzodiazepine-2,5-diones as Hdm2 antagonists.
PDB None

Name CHEMBL208625
Affinity IC50 = 11.6uM
Assay description Inhibition of p53-Hdm2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16647257
Doc title Novel 1,4-benzodiazepine-2,5-diones as Hdm2 antagonists with improved cellular activity.
PDB None

Name CHEMBL2089149
Affinity Ki = 1.37uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089150
Affinity Ki > 100uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089151
Affinity Ki = 0.721uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089152
Affinity Ki = 0.707uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089153
Affinity Ki > 100uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089200
Affinity Ki = 1.72uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089201
Affinity Ki = 0.315uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089202
Affinity Ki = 8.18uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089203
Affinity Ki = 0.091uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089204
Affinity Ki = 3.02uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089205
Affinity Ki > 100uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089206
Affinity Ki > 100uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089207
Affinity Ki = 37.7uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089208
Affinity Ki = 0.089uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089209
Affinity Ki = 1.05uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089210
Affinity Ki = 3.98uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089211
Affinity Ki = 0.518uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089320
Affinity Ki = 11.9uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089321
Affinity Ki > 100uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL2089322
Affinity Ki = 21.1uM
Assay description Inhibition of MDM2 binding domain assessed as inhibition of p53 binding to MDM2 after 1 hr by fluorescence polarization assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22940704
Doc title Structure-activity relationship and antitumor activity of thio-benzodiazepines as p53-MDM2 protein-protein interaction inhibitors.
PDB None

Name CHEMBL208999
Affinity IC50 = 2.7uM
Assay description Inhibition of p53-Hdm2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16647257
Doc title Novel 1,4-benzodiazepine-2,5-diones as Hdm2 antagonists with improved cellular activity.
PDB None

Name CHEMBL209156
Affinity IC50 = 10.2uM
Assay description Inhibition p53-HDM2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16600594
Doc title Enhanced pharmacokinetic properties of 1,4-benzodiazepine-2,5-dione antagonists of the HDM2-p53 protein-protein interaction through structure-based drug design.
PDB None

Name CHEMBL209169
Affinity IC50 = 0.51uM
Assay description Inhibition p53-HDM2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16600594
Doc title Enhanced pharmacokinetic properties of 1,4-benzodiazepine-2,5-dione antagonists of the HDM2-p53 protein-protein interaction through structure-based drug design.
PDB None

Name CHEMBL209201
Affinity IC50 = 2.7uM
Assay description Displacement of p53 mimicking fluorescent peptide from Hdm2 by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16630722
Doc title Enantiomerically pure 1,4-benzodiazepine-2,5-diones as Hdm2 antagonists.
PDB None

Name CHEMBL210151
Affinity IC50 = 0.7uM
Assay description Inhibition p53-HDM2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16600594
Doc title Enhanced pharmacokinetic properties of 1,4-benzodiazepine-2,5-dione antagonists of the HDM2-p53 protein-protein interaction through structure-based drug design.
PDB None

Name CHEMBL210151
Affinity IC50 = 0.856uM
Assay description Displacement of p53 mimicking fluorescent peptide from Hdm2 by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16630722
Doc title Enantiomerically pure 1,4-benzodiazepine-2,5-diones as Hdm2 antagonists.
PDB None

Name CHEMBL210409
Affinity IC50 = 2.82uM
Assay description Inhibition p53-HDM2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16600594
Doc title Enhanced pharmacokinetic properties of 1,4-benzodiazepine-2,5-dione antagonists of the HDM2-p53 protein-protein interaction through structure-based drug design.
PDB None

Name CHEMBL210443
Affinity IC50 = 3.33uM
Assay description Inhibition p53-HDM2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16600594
Doc title Enhanced pharmacokinetic properties of 1,4-benzodiazepine-2,5-dione antagonists of the HDM2-p53 protein-protein interaction through structure-based drug design.
PDB None

Name CHEMBL210545
Affinity IC50 = 0.394uM
Assay description Displacement of p53 mimicking fluorescent peptide from Hdm2 by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16630722
Doc title Enantiomerically pure 1,4-benzodiazepine-2,5-diones as Hdm2 antagonists.
PDB None

Name CHEMBL210545
Affinity IC50 = 0.7uM
Assay description Inhibition of p53-Hdm2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16647257
Doc title Novel 1,4-benzodiazepine-2,5-diones as Hdm2 antagonists with improved cellular activity.
PDB None

Name CHEMBL210611
Affinity Ki = 0.038uM
Assay description Displacement of PMDM6-F from human recombinant MDM2
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16759082
Doc title Structure-based design of spiro-oxindoles as potent, specific small-molecule inhibitors of the MDM2-p53 interaction.
PDB None

Name CHEMBL210778
Affinity Ki = 120nM
Assay description Binding affinity to MDM2
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16789731
Doc title Discovery of a nanomolar inhibitor of the human murine double minute 2 (MDM2)-p53 interaction through an integrated, virtual database screening strategy.
PDB None

Name CHEMBL210789
Affinity Ki = 0.044uM
Assay description Displacement of PMDM6-F from human recombinant MDM2
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16759082
Doc title Structure-based design of spiro-oxindoles as potent, specific small-molecule inhibitors of the MDM2-p53 interaction.
PDB None

Name CHEMBL210853
Affinity IC50 = 2.6uM
Assay description Inhibition of p53-Hdm2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16647257
Doc title Novel 1,4-benzodiazepine-2,5-diones as Hdm2 antagonists with improved cellular activity.
PDB None

Name CHEMBL210913
Affinity IC50 = 1.11uM
Assay description Inhibition p53-HDM2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16600594
Doc title Enhanced pharmacokinetic properties of 1,4-benzodiazepine-2,5-dione antagonists of the HDM2-p53 protein-protein interaction through structure-based drug design.
PDB None

Name CHEMBL211045
Affinity IC50 = 0.18uM
Assay description Inhibition of GST-tagged MDM2 binding to p53 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL212568
Affinity IC50 = 19uM
Assay description Inhibition of p53-Hdm2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16647257
Doc title Novel 1,4-benzodiazepine-2,5-diones as Hdm2 antagonists with improved cellular activity.
PDB None

Name CHEMBL212747
Affinity IC50 = 12.2uM
Assay description Inhibition of p53-Hdm2 interaction by FP assay
-- Direct protein complex subunits assigned: MDM2_HUMAN (Expert curation) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/16647257
Doc title Novel 1,4-benzodiazepine-2,5-diones as Hdm2 antagonists with improved cellular activity.
PDB None

Name CHEMBL2151052
Affinity IC50 = 7.1uM
Assay description Inhibition of GST-tagged MDM2 binding to p53 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL2152318
Affinity IC50 = 2.7uM
Assay description Inhibition of GST-tagged MDM2 binding to p53 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL2152319
Affinity IC50 = 1.8uM
Assay description Inhibition of GST-tagged MDM2 binding to p53 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL2152320
Affinity IC50 = 0.26uM
Assay description Inhibition of GST-tagged MDM2 binding to p53 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL2152321
Affinity IC50 = 3.1uM
Assay description Inhibition of GST-tagged MDM2 binding to p53 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL2152322
Affinity IC50 = 9.5uM
Assay description Inhibition of GST-tagged MDM2 binding to p53 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL2152323
Affinity IC50 = 3uM
Assay description Inhibition of GST-tagged MDM2 binding to p53 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL2152324
Affinity IC50 = 0.84uM
Assay description Inhibition of GST-tagged MDM2 binding to p53 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL2152325
Affinity IC50 = 4.4uM
Assay description Inhibition of GST-tagged MDM2 binding to p53 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL2152326
Affinity IC50 = 0.34uM
Assay description Inhibition of GST-tagged MDM2 binding to p53 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL2152327
Affinity IC50 = 1.1uM
Assay description Inhibition of GST-tagged MDM2 binding to p53 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL2152328
Affinity IC50 = 1.8uM
Assay description Inhibition of GST-tagged MDM2 binding to p53 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL2152329
Affinity IC50 = 1.2uM
Assay description Inhibition of GST-tagged MDM2 binding to p53 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL2152330
Affinity IC50 = 0.14uM
Assay description Inhibition of GST-tagged MDM2 binding to p53 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL2152331
Affinity IC50 = 11.9uM
Assay description Inhibition of GST-tagged MDM2 binding to p53 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL2152331
Affinity IC50 = 0.16uM
Assay description Inhibition of GST-tagged MDM2 binding to p53 by ELISA
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL2152332
Affinity IC50 = 13.6uM
Assay description Inhibition of MDM2 binding to p53
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22995624
Doc title Discovery of novel dihydroimidazothiazole derivatives as p53-MDM2 protein-protein interaction inhibitors: synthesis, biological evaluation and structure-activity relationships.
PDB None

Name CHEMBL2171149
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171150
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171151
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171152
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171153
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171154
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171155
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171156
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171157
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171158
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171159
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171160
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171161
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171162
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171163
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171164
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171165
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171166
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171167
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171336
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171337
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171338
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171339
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171340
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171341
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171342
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171343
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171344
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171345
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171346
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171347
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2171348
Affinity Not Active
Assay description Inhibition of wild type Mdm2 at 20 ug/mL after 2 hrs by cell-based luciferase assay
-- Homologous single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/22832321
Doc title Design and synthesis of Pictet-Spengler condensation products that exhibit oncogenic-RAS synthetic lethality and induce non-apoptotic cell death.
PDB None

Name CHEMBL2177203
Affinity IC50 = 0.076uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB 2LZG - (13Q)

Name CHEMBL2177203
Affinity IC50 = 25.2uM
Assay description Inhibition of MDM2 in human SJSA1 cells assessed as upregulation of p21 transcript level after 7 hrs by qRT-PCR analysis in presence of 10% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB 2LZG - (13Q)

Name CHEMBL2177203
Affinity IC50 = 7.1uM
Assay description Inhibition of MDM2-induced proliferation of human SJSA1 cells assessed as EdU incorporation by High content screening assay in presence of 10% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB 2LZG - (13Q)

Name CHEMBL2177203
Affinity IC50 = 1.1uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB 2LZG - (13Q)

Name CHEMBL2177813
Affinity IC50 = 0.034uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB 2LZG - (13Q)

Name CHEMBL2177813
Affinity IC50 = 3.4uM
Assay description Inhibition of MDM2-induced proliferation of human SJSA1 cells assessed as EdU incorporation by High content screening assay in presence of 10% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB 2LZG - (13Q)

Name CHEMBL2177813
Affinity IC50 = 18.1uM
Assay description Inhibition of MDM2 in human SJSA1 cells assessed as upregulation of p21 transcript level after 7 hrs by qRT-PCR analysis in presence of 10% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB 2LZG - (13Q)

Name CHEMBL2177813
Affinity IC50 = 0.36uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB 2LZG - (13Q)

Name CHEMBL223723
Affinity IC50 = 13.1uM
Assay description Inhibition of GST-tagged HDM2 (unknown origin) assessed as p53 ubiquitination by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24113239
Doc title 5-Deazaflavin derivatives as inhibitors of p53 ubiquitination by HDM2.
PDB None

Name CHEMBL224408
Affinity IC50 > 100uM
Assay description Inhibition of GST-tagged HDM2 (unknown origin) assessed as p53 ubiquitination by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24113239
Doc title 5-Deazaflavin derivatives as inhibitors of p53 ubiquitination by HDM2.
PDB None

Name CHEMBL225454
Affinity IC50 = 19.6uM
Assay description Inhibition of GST-tagged HDM2 (unknown origin) assessed as p53 ubiquitination by Western blot analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/24113239
Doc title 5-Deazaflavin derivatives as inhibitors of p53 ubiquitination by HDM2.
PDB None

Name CHEMBL2347375
Affinity IC50 = 21.3nM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB 3LBL - (MI6)

Name CHEMBL2347375
Affinity IC50 = 9.4nM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB 3LBL - (MI6)

Name CHEMBL2347376
Affinity IC50 = 5uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347376
Affinity IC50 = 0.29uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347376
Affinity IC50 = 16.7uM
Assay description Inhibition of MDM2-induced proliferation of human SJSA1 cells assessed as EdU incorporation by High content screening assay in presence of 10% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347377
Affinity IC50 = 2.8uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347377
Affinity IC50 = 0.14uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347377
Affinity IC50 = 15.3uM
Assay description Inhibition of MDM2-induced proliferation of human SJSA1 cells assessed as EdU incorporation by High content screening assay in presence of 10% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347378
Affinity IC50 = 27.5uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB 2LZG - (13Q)

Name CHEMBL2347378
Affinity IC50 = 2.9uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB 2LZG - (13Q)

Name CHEMBL2347379
Affinity IC50 > 30uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB 2LZG - (13Q)

Name CHEMBL2347379
Affinity IC50 = 8.1uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB 2LZG - (13Q)

Name CHEMBL2347380
Affinity IC50 = 1.8uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347381
Affinity IC50 = 1.9uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347382
Affinity IC50 = 2.1uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347383
Affinity IC50 = 20.7uM
Assay description Inhibition of MDM2 in human SJSA1 cells assessed as upregulation of p21 transcript level after 7 hrs by qRT-PCR analysis in presence of 10% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347383
Affinity IC50 = 5.9uM
Assay description Inhibition of MDM2-induced proliferation of human SJSA1 cells assessed as EdU incorporation by High content screening assay in presence of 10% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347383
Affinity IC50 = 0.086uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347383
Affinity IC50 = 2.9uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347384
Affinity IC50 > 30uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347384
Affinity IC50 = 11.7uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347385
Affinity IC50 > 30uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347385
Affinity IC50 = 1.8uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347386
Affinity IC50 > 30uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347386
Affinity IC50 > 30uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347387
Affinity IC50 = 1.4uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347387
Affinity IC50 > 30uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347388
Affinity IC50 = 14.4uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347388
Affinity IC50 = 0.88uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347389
Affinity IC50 = 5.7uM
Assay description Inhibition of MDM2-induced proliferation of human SJSA1 cells assessed as EdU incorporation by High content screening assay in presence of 10% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347389
Affinity IC50 = 0.3uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347389
Affinity IC50 = 29.4uM
Assay description Inhibition of MDM2 in human SJSA1 cells assessed as upregulation of p21 transcript level after 7 hrs by qRT-PCR analysis in presence of 10% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347389
Affinity Active
Assay description Inhibition of MDM2-induced proliferation of human HCT116 cells expressing wild type p53 assessed as BrdU incorporation after 16 hrs by flow cytometric analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347389
Affinity IC50 = 0.1uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347389
Affinity Active
Assay description Inhibition of MDM2 in human HCT116 cells expressing wild type p53 assessed as upregulation of p21 mRNA level after 7 hrs by RT-PCR analysis
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347389
Affinity IC50 = 15.7uM
Assay description Inhibition of MDM2-induced proliferation of human SJSA1 cells assessed as EdU incorporation by High content screening assay in presence of 10% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347389
Affinity IC50 = 4.5uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347389
Affinity IC50 = 1.7uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347390
Affinity IC50 = 9.9uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347390
Affinity IC50 > 30uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347391
Affinity IC50 = 15.2uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay in presence of 15% serum
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347391
Affinity IC50 = 1.3uM
Assay description Inhibition of human recombinant GST-thrombin-MDM2 (1 to 188) expressed in Escherichia coli assessed as reduction of MDM2-p53 interaction incubated for 20 mins followed by p53 addition measured after 60 mins by HTRF assay
-- Direct single protein target assigned: MDM2_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23597064
Doc title Rational design and binding mode duality of MDM2-p53 inhibitors.
PDB None

Name CHEMBL2347392
Affinity IC50 > 30uM
Assay description Inhibition of human recombinan