TNFa

Tumor necrosis factor (tnf-alpha)

Reviewed UniProt entries for TNFa and its protein partners


UniProt code UniProt Name Protein Name Gene Name Organism Length
P59684 TNFA_BOSIN Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Bos indicus (Zebu) 234
Q1G1A2 TNFA_BOSTR Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Boselaphus tragocamelus (Nilgai) 234
Q06599 TNFA_BOVIN Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Bos taurus (Bovine) 234
P59693 TNFA_BUBBU Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Bubalus bubalis (Domestic water buffalo) 233
Q2MH05 TNFA_BUBCA Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Bubalus carabanensis (Swamp type water buffalo) (Bubalus bubalis carabanensis) 234
Q19LH4 TNFA_CALJA Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Callithrix jacchus (White-tufted-ear marmoset) 232
Q75N23 TNFA_CAMBA Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Camelus bactrianus (Bactrian camel) 233
P51742 TNFA_CANFA Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Canis familiaris (Dog) (Canis lupus familiaris) 233
P13296 TNFA_CAPHI Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Capra hircus (Goat) 234
P51435 TNFA_CAVPO Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Cavia porcellus (Guinea pig) 234
P51743 TNFA_CEREL Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] (Fragment) TNF TNFA TNFSF2 Cervus elaphus (Red deer) 229
Q1WM27 TNFA_DASNO Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Dasypus novemcinctus (Nine-banded armadillo) 233
Q8WNR1 TNFA_DELLE Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Delphinapterus leucas (Beluga whale) 233
P19101 TNFA_FELCA Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Felis catus (Cat) (Felis silvestris catus) 233
P29553 TNFA_HORSE Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Equus caballus (Horse) 234
P01375 TNFA_HUMAN Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Homo sapiens (Human) 233
P59694 TNFA_LAMGL Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Lama glama (Llama) 233
O77764 TNFA_MACEU Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Macropus eugenii (Tammar wallaby) 233
P79337 TNFA_MACFA Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) 233
P48094 TNFA_MACMU Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Macaca mulatta (Rhesus macaque) 233
O35734 TNFA_MARMO Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Marmota monax (Woodchuck) 233
Q8HZD9 TNFA_PANTR Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Pan troglodytes (Chimpanzee) 232
P59695 TNFA_PAPAN Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Papio anubis (Olive baboon) 233
O77510 TNFA_PAPHU Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Papio hamadryas ursinus (Chacma baboon) 233
P33620 TNFA_PAPSP Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Papio sp. (Baboon) 233
P36939 TNFA_PERLE Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Peromyscus leucopus (White-footed mouse) 235
P23563 TNFA_PIG Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Sus scrofa (Pig) 232
P04924 TNFA_RABIT Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Oryctolagus cuniculus (Rabbit) 235
P16599 TNFA_RAT Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] Tnf Tnfa Tnfsf2 Rattus norvegicus (Rat) 235
Q8MKG8 TNFA_SAISC Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Saimiri sciureus (Common squirrel monkey) 233
P23383 TNFA_SHEEP Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Ovis aries (Sheep) 234
Q8JFG3 TNFA_SPAAU Tumor necrosis factor (TNF-alpha) tnf tnfa Sparus aurata (Gilthead sea bream) 253
P79374 TNFA_TRIVU Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Trichosurus vulpecula (Brush-tailed possum) 233
Q539C2 TNFA_TUPTA Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Tupaia tana (Large tree shrew) 234
Q9BEA1 TNFA_TURTR Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Tursiops truncatus (Atlantic bottle-nosed dolphin) (Delphinus truncatus) 233
P06804 TNFA_MOUSE Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] Tnf Tnfa Tnfsf2 Mus musculus (Mouse) 235

Small molecules tested in TNFa binding assays:

25 datapoints for 24 distinct small molecules


Name Sci310_1
Affinity IC50 (ELISA) 22uM
Assay description An ELISA was used to measure inhibition of solution-phase TNF-R1 horseradish peroxidase conjugate binding to biotinylated TNF-a immobilized on a strepavidin-coated microtiter plate by serial dilutions of compound
-- Direct single protein target assigned: TNFA_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1126/science.1116304
Doc title Small-molecule inhibition of TNF-alpha.
PDB 2AZ5 - (307)

Name CHEMBL1269862
Affinity INH = 64%
Assay description Inhibition of TNFalpha in LPS-stimulated mouse RAW264.7 cells at 10 uM after 2 hrs treated after 14 hrs of LPS challenge by FACS analysis
-- Direct single protein target assigned: TNFA_MOUSE (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20850306
Doc title Discovery of the inhibitors of tumor necrosis factor alpha with structure-based virtual screening.
PDB None

Name CHEMBL1269967
Affinity INH = 54.6%
Assay description Inhibition of TNFalpha in LPS-stimulated mouse RAW264.7 cells at 10 uM after 2 hrs treated after 14 hrs of LPS challenge by FACS analysis
-- Direct single protein target assigned: TNFA_MOUSE (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20850306
Doc title Discovery of the inhibitors of tumor necrosis factor alpha with structure-based virtual screening.
PDB None

Name CHEMBL1269968
Affinity INH = 47.9%
Assay description Inhibition of TNFalpha in LPS-stimulated mouse RAW264.7 cells at 10 uM after 2 hrs treated after 14 hrs of LPS challenge by FACS analysis
-- Direct single protein target assigned: TNFA_MOUSE (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20850306
Doc title Discovery of the inhibitors of tumor necrosis factor alpha with structure-based virtual screening.
PDB None

Name CHEMBL1270078
Affinity INH = 52.6%
Assay description Inhibition of TNFalpha in LPS-stimulated mouse RAW264.7 cells at 10 uM after 2 hrs treated after 14 hrs of LPS challenge by FACS analysis
-- Direct single protein target assigned: TNFA_MOUSE (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20850306
Doc title Discovery of the inhibitors of tumor necrosis factor alpha with structure-based virtual screening.
PDB None

Name CHEMBL1270079
Affinity INH = 45.8%
Assay description Inhibition of TNFalpha in LPS-stimulated mouse RAW264.7 cells at 10 uM after 2 hrs treated after 14 hrs of LPS challenge by FACS analysis
-- Direct single protein target assigned: TNFA_MOUSE (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20850306
Doc title Discovery of the inhibitors of tumor necrosis factor alpha with structure-based virtual screening.
PDB None

Name CHEMBL2337218
Affinity Not Active
Assay description Inhibition of TNFalpha (unknown origin)-induced NF-kappaB activation in mouse NIH/3T3 cells assessed as inhibition of NF-kappaB binding to DNA at 20 uM after 6 hrs by luciferase reporter gene assay
-- Direct single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23357309
Doc title Microfluidics assisted synthesis and bioevaluation of sinomenine derivatives as antiinflammatory agents.
PDB None

Name CHEMBL2337219
Affinity Active
Assay description Inhibition of TNFalpha (unknown origin)-induced NF-kappaB activation in mouse NIH/3T3 cells assessed as inhibition of NF-kappaB binding to DNA at 20 uM after 6 hrs by luciferase reporter gene assay
-- Direct single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23357309
Doc title Microfluidics assisted synthesis and bioevaluation of sinomenine derivatives as antiinflammatory agents.
PDB None

Name CHEMBL2337609
Affinity Active
Assay description Inhibition of TNFalpha (unknown origin)-induced NF-kappaB activation in mouse NIH/3T3 cells assessed as inhibition of NF-kappaB binding to DNA at 20 uM after 6 hrs by luciferase reporter gene assay
-- Direct single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23357309
Doc title Microfluidics assisted synthesis and bioevaluation of sinomenine derivatives as antiinflammatory agents.
PDB None

Name CHEMBL2337610
Affinity Not Active
Assay description Inhibition of TNFalpha (unknown origin)-induced NF-kappaB activation in mouse NIH/3T3 cells assessed as inhibition of NF-kappaB binding to DNA at 20 uM after 6 hrs by luciferase reporter gene assay
-- Direct single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23357309
Doc title Microfluidics assisted synthesis and bioevaluation of sinomenine derivatives as antiinflammatory agents.
PDB None

Name CHEMBL2337611
Affinity Active
Assay description Inhibition of TNFalpha (unknown origin)-induced NF-kappaB activation in mouse NIH/3T3 cells assessed as inhibition of NF-kappaB binding to DNA at 20 uM after 6 hrs by luciferase reporter gene assay
-- Direct single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23357309
Doc title Microfluidics assisted synthesis and bioevaluation of sinomenine derivatives as antiinflammatory agents.
PDB None

Name CHEMBL2337612
Affinity Not Active
Assay description Inhibition of TNFalpha (unknown origin)-induced NF-kappaB activation in mouse NIH/3T3 cells assessed as inhibition of NF-kappaB binding to DNA at 20 uM after 6 hrs by luciferase reporter gene assay
-- Direct single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23357309
Doc title Microfluidics assisted synthesis and bioevaluation of sinomenine derivatives as antiinflammatory agents.
PDB None

Name CHEMBL2337613
Affinity Active
Assay description Inhibition of TNFalpha (unknown origin)-induced NF-kappaB activation in mouse NIH/3T3 cells assessed as inhibition of NF-kappaB binding to DNA at 20 uM after 6 hrs by luciferase reporter gene assay
-- Direct single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23357309
Doc title Microfluidics assisted synthesis and bioevaluation of sinomenine derivatives as antiinflammatory agents.
PDB None

Name CHEMBL2337614
Affinity Not Active
Assay description Inhibition of TNFalpha (unknown origin)-induced NF-kappaB activation in mouse NIH/3T3 cells assessed as inhibition of NF-kappaB binding to DNA at 20 uM after 6 hrs by luciferase reporter gene assay
-- Direct single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23357309
Doc title Microfluidics assisted synthesis and bioevaluation of sinomenine derivatives as antiinflammatory agents.
PDB None

Name CHEMBL2337615
Affinity Active
Assay description Inhibition of TNFalpha (unknown origin)-induced NF-kappaB activation in mouse NIH/3T3 cells assessed as inhibition of NF-kappaB binding to DNA at 20 uM after 6 hrs by luciferase reporter gene assay
-- Direct single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23357309
Doc title Microfluidics assisted synthesis and bioevaluation of sinomenine derivatives as antiinflammatory agents.
PDB None

Name CHEMBL2337616
Affinity Active
Assay description Inhibition of TNFalpha (unknown origin)-induced NF-kappaB activation in mouse NIH/3T3 cells assessed as inhibition of NF-kappaB binding to DNA at 20 uM after 6 hrs by luciferase reporter gene assay
-- Direct single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23357309
Doc title Microfluidics assisted synthesis and bioevaluation of sinomenine derivatives as antiinflammatory agents.
PDB None

Name CHEMBL2337617
Affinity Active
Assay description Inhibition of TNFalpha (unknown origin)-induced NF-kappaB activation in mouse NIH/3T3 cells assessed as inhibition of NF-kappaB binding to DNA at 20 uM after 6 hrs by luciferase reporter gene assay
-- Direct single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23357309
Doc title Microfluidics assisted synthesis and bioevaluation of sinomenine derivatives as antiinflammatory agents.
PDB None

Name CHEMBL2337619
Affinity Active
Assay description Inhibition of TNFalpha (unknown origin)-induced NF-kappaB activation in mouse NIH/3T3 cells assessed as inhibition of NF-kappaB binding to DNA at 20 uM after 6 hrs by luciferase reporter gene assay
-- Direct single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23357309
Doc title Microfluidics assisted synthesis and bioevaluation of sinomenine derivatives as antiinflammatory agents.
PDB None

Name CHEMBL2337623
Affinity Active
Assay description Inhibition of TNFalpha (unknown origin)-induced NF-kappaB activation in mouse NIH/3T3 cells assessed as inhibition of NF-kappaB binding to DNA at 20 uM after 6 hrs by luciferase reporter gene assay
-- Direct single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23357309
Doc title Microfluidics assisted synthesis and bioevaluation of sinomenine derivatives as antiinflammatory agents.
PDB None

Name CHEMBL2337626
Affinity Active
Assay description Inhibition of TNFalpha (unknown origin)-induced NF-kappaB activation in mouse NIH/3T3 cells assessed as inhibition of NF-kappaB binding to DNA at 20 uM after 6 hrs by luciferase reporter gene assay
-- Direct single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23357309
Doc title Microfluidics assisted synthesis and bioevaluation of sinomenine derivatives as antiinflammatory agents.
PDB None

Name CHEMBL2337627
Affinity Active
Assay description Inhibition of TNFalpha (unknown origin)-induced NF-kappaB activation in mouse NIH/3T3 cells assessed as inhibition of NF-kappaB binding to DNA at 20 uM after 6 hrs by luciferase reporter gene assay
-- Direct single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23357309
Doc title Microfluidics assisted synthesis and bioevaluation of sinomenine derivatives as antiinflammatory agents.
PDB None

Name CHEMBL2337628
Affinity Not Active
Assay description Inhibition of TNFalpha (unknown origin)-induced NF-kappaB activation in mouse NIH/3T3 cells assessed as inhibition of NF-kappaB binding to DNA at 20 uM after 6 hrs by luciferase reporter gene assay
-- Direct single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23357309
Doc title Microfluidics assisted synthesis and bioevaluation of sinomenine derivatives as antiinflammatory agents.
PDB None

Name CHEMBL248095
Affinity Not Active
Assay description Inhibition of TNFalpha (unknown origin)-induced NF-kappaB activation in mouse NIH/3T3 cells assessed as inhibition of NF-kappaB binding to DNA at 20 uM after 6 hrs by luciferase reporter gene assay
-- Direct single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/23357309
Doc title Microfluidics assisted synthesis and bioevaluation of sinomenine derivatives as antiinflammatory agents.
PDB None

Name CHEMBL255489
Affinity Kd = 13uM
Assay description Binding affinity to TNF
-- Homologous single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/18075579
Doc title Reaching for high-hanging fruit in drug discovery at protein-protein interfaces.
PDB None

Name CHEMBL8260
Affinity INH = 71.3%
Assay description Inhibition of TNFalpha in LPS-stimulated mouse RAW264.7 cells at 10 uM after 2 hrs treated after 14 hrs of LPS challenge by FACS analysis
-- Direct single protein target assigned: TNFA_MOUSE (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20850306
Doc title Discovery of the inhibitors of tumor necrosis factor alpha with structure-based virtual screening.
PDB None

15 PDB entries for TNFa and its protein partners


1A8M
Crystal structure of TNF-alpha mutant R31D with greater affinity for receptor R1 compared with R2.

Entity type Code Description
polypeptide(L) P01375 TUMOR NECROSIS FACTOR ALPHA

1TNF
The structure of tumor necrosis factor-alpha at 2.6 A resolution. Implications for receptor binding.

Entity type Code Description
polypeptide(L) P01375 TUMOR NECROSIS FACTOR-ALPHA

2AZ5
Small-molecule inhibition of TNF-alpha.

Entity type Code Description
polypeptide(L) P01375 Tumor necrosis factor (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) (Cachectin) [Contains: Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form]
Ligand 307 6,7-DIMETHYL-3-[(METHYL{2-[METHYL({1-[3-(TRIFLUOROMETHYL)PHENYL]-1H-INDOL-3-YL}METHYL)AMINO]ETHYL}AMINO)METHYL]-4H-CHROMEN-4-ONE

2E7A
Creation and X-ray structure analysis of the tumor necrosis factor receptor-1-selective mutant of a tumor necrosis factor-alpha antagonist

Entity type Code Description
polypeptide(L) P01375 Tumor necrosis factor

2TNF
The structure of mouse tumour-necrosis factor at 1.4 A resolution: towards modulation of its selectivity and trimerization.

Entity type Code Description
polypeptide(L) P06804 PROTEIN (TUMOR NECROSIS FACTOR ALPHA)
Ligand TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL

2TUN
Conformational Changes in the (Ala-84-Val) Mutant of Tumor Necrosis Factor

Entity type Code Description
polypeptide(L) P01375 TUMOR NECROSIS FACTOR-ALPHA

2ZJC
Structure-Function Relationship of Tumor Necrosis Factor (TNF) and Its Receptor Interaction Based on 3D Structural Analysis of a Fully Active TNFR1-Selective TNF Mutant

Entity type Code Description
polypeptide(L) P01375 Tumor necrosis factor

2ZPX
Fast binding kinetics and conserved 3D structure underlie the antagonistic activity of mutant TNF: useful information for designing artificial proteo-antagonists

Entity type Code Description
polypeptide(L) P01375 Tumor necrosis factor

3ALQ
Solution of the Structure of the TNF-TNFR2 Complex

Entity type Code Description
polypeptide(L) P01375 Tumor necrosis factor
polypeptide(L) P20333 Tumor necrosis factor receptor superfamily member 1B

3IT8
Crystal structure of TNFa complexed with a poxvirus MHC-related TNF binding protein

Entity type Code Description
polypeptide(L) P01375 Tumor necrosis factor
polypeptide(L) Q9DHW0 2L protein
Ligand NAG SUGAR (N-ACETYL-D-GLUCOSAMINE)

3L9J
Selection of a novel and highly specific TNF{alpha} antagonist: insight from the crystal structure of the antagonist-TNF{alpha} complex

Entity type Code Description
polypeptide(L) 3L9J TNFalpha
polypeptide(L) P01375 Tumor necrosis factor, soluble form

3WD5
Comparison of the inhibition mechanisms of Adalimumab and Infliximab in treating TNFalpha-associated diseases from a molecular view

Entity type Code Description
polypeptide(L) P01375 Tumor necrosis factor
polypeptide(L) 3WD5 Adalimumab Light Chain
polypeptide(L) 3WD5 Adalimumab Heavy Chain

4G3Y
Structural basis for treating tumor necrosis factor alpha (TNFalpha)-associated diseases with the therapeutic antibody infliximab

Entity type Code Description
polypeptide(L) 4G3Y infliximab Fab L
polypeptide(L) 4G3Y infliximab Fab H
polypeptide(L) P01375 Tumor necrosis factor

4TSV
High resolution crystal structure of a human tumor necrosis factor-alpha mutant with low systemic toxicity.

Entity type Code Description
polypeptide(L) P01375 TUMOR NECROSIS FACTOR-ALPHA

5TSW
High resolution crystal structure of a human tumor necrosis factor-alpha mutant with low systemic toxicity.

Entity type Code Description
polypeptide(L) P01375 PROTEIN (TUMOR NECROSIS FACTOR-ALPHA)