TNFa

Tumor necrosis factor (tnf-alpha)

Reviewed UniProt entries for TNFa and its protein partners


UniProt code UniProt Name Protein Name Gene Name Organism Length
P59684 TNFA_BOSIN Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Bos indicus (Zebu) 234
Q1G1A2 TNFA_BOSTR Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Boselaphus tragocamelus (Nilgai) 234
Q06599 TNFA_BOVIN Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Bos taurus (Bovine) 234
P59693 TNFA_BUBBU Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Bubalus bubalis (Domestic water buffalo) 233
Q2MH05 TNFA_BUBCA Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Bubalus carabanensis (Swamp type water buffalo) (Bubalus bubalis carabanensis) 234
Q19LH4 TNFA_CALJA Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Callithrix jacchus (White-tufted-ear marmoset) 232
Q75N23 TNFA_CAMBA Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Camelus bactrianus (Bactrian camel) 233
P51742 TNFA_CANFA Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Canis familiaris (Dog) (Canis lupus familiaris) 233
P13296 TNFA_CAPHI Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Capra hircus (Goat) 234
P51435 TNFA_CAVPO Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Cavia porcellus (Guinea pig) 234
P51743 TNFA_CEREL Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] (Fragment) TNF TNFA TNFSF2 Cervus elaphus (Red deer) 229
Q1WM27 TNFA_DASNO Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Dasypus novemcinctus (Nine-banded armadillo) 233
Q8WNR1 TNFA_DELLE Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Delphinapterus leucas (Beluga whale) 233
P19101 TNFA_FELCA Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Felis catus (Cat) (Felis silvestris catus) 233
P29553 TNFA_HORSE Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Equus caballus (Horse) 234
P01375 TNFA_HUMAN Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Homo sapiens (Human) 233
P59694 TNFA_LAMGL Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Lama glama (Llama) 233
O77764 TNFA_MACEU Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Macropus eugenii (Tammar wallaby) 233
P79337 TNFA_MACFA Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) 233
P48094 TNFA_MACMU Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Macaca mulatta (Rhesus macaque) 233
O35734 TNFA_MARMO Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Marmota monax (Woodchuck) 233
Q8HZD9 TNFA_PANTR Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Pan troglodytes (Chimpanzee) 232
P59695 TNFA_PAPAN Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Papio anubis (Olive baboon) 233
O77510 TNFA_PAPHU Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Papio hamadryas ursinus (Chacma baboon) 233
P33620 TNFA_PAPSP Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Papio sp. (Baboon) 233
P36939 TNFA_PERLE Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Peromyscus leucopus (White-footed mouse) 235
P23563 TNFA_PIG Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Sus scrofa (Pig) 232
P04924 TNFA_RABIT Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Oryctolagus cuniculus (Rabbit) 235
P16599 TNFA_RAT Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] Tnf Tnfa Tnfsf2 Rattus norvegicus (Rat) 235
Q8MKG8 TNFA_SAISC Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Saimiri sciureus (Common squirrel monkey) 233
P23383 TNFA_SHEEP Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Ovis aries (Sheep) 234
Q8JFG3 TNFA_SPAAU Tumor necrosis factor (TNF-alpha) tnf tnfa Sparus aurata (Gilthead sea bream) 253
P79374 TNFA_TRIVU Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Trichosurus vulpecula (Brush-tailed possum) 233
Q539C2 TNFA_TUPTA Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Tupaia tana (Large tree shrew) 234
Q9BEA1 TNFA_TURTR Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] TNF TNFA TNFSF2 Tursiops truncatus (Atlantic bottle-nosed dolphin) (Delphinus truncatus) 233
P06804 TNFA_MOUSE Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor; membrane form; Tumor necrosis factor; soluble form] Tnf Tnfa Tnfsf2 Mus musculus (Mouse) 235

Small molecules tested in TNFa binding assays:

8 datapoints for 7 distinct small molecules


Name Sci310_1
Affinity IC50 (ELISA) 22uM
Assay description An ELISA was used to measure inhibition of solution-phase TNF-R1 horseradish peroxidase conjugate binding to biotinylated TNF-a immobilized on a strepavidin-coated microtiter plate by serial dilutions of compound
-- Direct single protein target assigned: TNFA_HUMAN (Expert curation) --
DOI http://dx.doi.org/10.1126/science.1116304
Doc title Small-molecule inhibition of TNF-alpha.
PDB 2AZ5 - (307)

Name CHEMBL1269862
Affinity INH = 64%
Assay description Inhibition of TNFalpha in LPS-stimulated mouse RAW264.7 cells at 10 uM after 2 hrs treated after 14 hrs of LPS challenge by FACS analysis
-- Direct single protein target assigned: TNFA_MOUSE (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20850306
Doc title Discovery of the inhibitors of tumor necrosis factor alpha with structure-based virtual screening.
PDB None

Name CHEMBL1269967
Affinity INH = 54.6%
Assay description Inhibition of TNFalpha in LPS-stimulated mouse RAW264.7 cells at 10 uM after 2 hrs treated after 14 hrs of LPS challenge by FACS analysis
-- Direct single protein target assigned: TNFA_MOUSE (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20850306
Doc title Discovery of the inhibitors of tumor necrosis factor alpha with structure-based virtual screening.
PDB None

Name CHEMBL1269968
Affinity INH = 47.9%
Assay description Inhibition of TNFalpha in LPS-stimulated mouse RAW264.7 cells at 10 uM after 2 hrs treated after 14 hrs of LPS challenge by FACS analysis
-- Direct single protein target assigned: TNFA_MOUSE (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20850306
Doc title Discovery of the inhibitors of tumor necrosis factor alpha with structure-based virtual screening.
PDB None

Name CHEMBL1270078
Affinity INH = 52.6%
Assay description Inhibition of TNFalpha in LPS-stimulated mouse RAW264.7 cells at 10 uM after 2 hrs treated after 14 hrs of LPS challenge by FACS analysis
-- Direct single protein target assigned: TNFA_MOUSE (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20850306
Doc title Discovery of the inhibitors of tumor necrosis factor alpha with structure-based virtual screening.
PDB None

Name CHEMBL1270079
Affinity INH = 45.8%
Assay description Inhibition of TNFalpha in LPS-stimulated mouse RAW264.7 cells at 10 uM after 2 hrs treated after 14 hrs of LPS challenge by FACS analysis
-- Direct single protein target assigned: TNFA_MOUSE (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20850306
Doc title Discovery of the inhibitors of tumor necrosis factor alpha with structure-based virtual screening.
PDB None

Name CHEMBL255489
Affinity Kd = 13uM
Assay description Binding affinity to TNF
-- Homologous single protein target assigned: TNFA_HUMAN (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/18075579
Doc title Reaching for high-hanging fruit in drug discovery at protein-protein interfaces.
PDB None

Name CHEMBL8260
Affinity INH = 71.3%
Assay description Inhibition of TNFalpha in LPS-stimulated mouse RAW264.7 cells at 10 uM after 2 hrs treated after 14 hrs of LPS challenge by FACS analysis
-- Direct single protein target assigned: TNFA_MOUSE (Autocuration) --
DOI http://www.ncbi.nlm.nih.gov/pubmed/20850306
Doc title Discovery of the inhibitors of tumor necrosis factor alpha with structure-based virtual screening.
PDB None

13 PDB entries for TNFa and its protein partners


1A8M
Crystal structure of TNF-alpha mutant R31D with greater affinity for receptor R1 compared with R2.

Entity type Code Description
polypeptide(L) P01375 TUMOR NECROSIS FACTOR ALPHA

1TNF
The structure of tumor necrosis factor-alpha at 2.6 A resolution. Implications for receptor binding.

Entity type Code Description
polypeptide(L) P01375 TUMOR NECROSIS FACTOR-ALPHA

2AZ5
Small-molecule inhibition of TNF-alpha.

Entity type Code Description
polypeptide(L) P01375 Tumor necrosis factor (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) (Cachectin) [Contains: Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form]
Ligand 307 6,7-DIMETHYL-3-[(METHYL{2-[METHYL({1-[3-(TRIFLUOROMETHYL)PHENYL]-1H-INDOL-3-YL}METHYL)AMINO]ETHYL}AMINO)METHYL]-4H-CHROMEN-4-ONE

2E7A
Creation and X-ray structure analysis of the tumor necrosis factor receptor-1-selective mutant of a tumor necrosis factor-alpha antagonist

Entity type Code Description
polypeptide(L) P01375 Tumor necrosis factor

2TNF
The structure of mouse tumour-necrosis factor at 1.4 A resolution: towards modulation of its selectivity and trimerization.

Entity type Code Description
polypeptide(L) P06804 PROTEIN (TUMOR NECROSIS FACTOR ALPHA)
Ligand TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL

2TUN
Conformational Changes in the (Ala-84-Val) Mutant of Tumor Necrosis Factor

Entity type Code Description
polypeptide(L) P01375 TUMOR NECROSIS FACTOR-ALPHA

2ZJC
Structure-Function Relationship of Tumor Necrosis Factor (TNF) and Its Receptor Interaction Based on 3D Structural Analysis of a Fully Active TNFR1-Selective TNF Mutant

Entity type Code Description
polypeptide(L) P01375 Tumor necrosis factor

2ZPX
Fast binding kinetics and conserved 3D structure underlie the antagonistic activity of mutant TNF: useful information for designing artificial proteo-antagonists

Entity type Code Description
polypeptide(L) P01375 Tumor necrosis factor

3ALQ
Solution of the Structure of the TNF-TNFR2 Complex

Entity type Code Description
polypeptide(L) P01375 Tumor necrosis factor
polypeptide(L) P20333 Tumor necrosis factor receptor superfamily member 1B

3IT8
Crystal structure of TNFa complexed with a poxvirus MHC-related TNF binding protein

Entity type Code Description
polypeptide(L) P01375 Tumor necrosis factor
polypeptide(L) Q9DHW0 2L protein
Ligand NAG SUGAR (N-ACETYL-D-GLUCOSAMINE)

3L9J
Selection of a novel and highly specific TNF{alpha} antagonist: insight from the crystal structure of the antagonist-TNF{alpha} complex

Entity type Code Description
polypeptide(L) 3L9J TNFalpha
polypeptide(L) P01375 Tumor necrosis factor, soluble form

4TSV
High resolution crystal structure of a human tumor necrosis factor-alpha mutant with low systemic toxicity.

Entity type Code Description
polypeptide(L) P01375 TUMOR NECROSIS FACTOR-ALPHA

5TSW
High resolution crystal structure of a human tumor necrosis factor-alpha mutant with low systemic toxicity.

Entity type Code Description
polypeptide(L) P01375 PROTEIN (TUMOR NECROSIS FACTOR-ALPHA)