SDM requires a PDB file of the wild-type protein structure in order to calculate a stability score for mutant proteins. If you do not have a PDB structure ready for input, the following two search boxes will help you to identify a relevant PDB structure.

Search the PDB for your protein of interest Type a protein name or keyword below to retrieve all PDB structures matching your search terms:

Search the PDB for your sequence of interest Paste an amino acid sequence:

Predicting the effect of mutations on protein stability
You have the option of either:
1. Uploading your own wild-type PDB file:	(max. 2 MB)
2. Or, you may enter a 4 digit PDB code:
You have the option of either:
1. Uploading your own mutant PDB file: Please note that this structure must match the wild-type structure exactly except for the mutant amino acid i.e. the structures must be the same length	(max. 2 MB)
2. Or, you may use our program, Andante, for building a mutant structure.
You must also enter the following information to run SDM:
1 letter code of PDB chain:
1 letter code of mutant residue:
Residue position (according to wild-type PDB file):

Alternatively, view example SDM output